The presence of bone morphogenetic proteins in demineralized freeze-dried bone allograft (DFDBA) are responsible for developing hard tissues in intraosseous defects. The most common mode of sterilization of bone allografts, i.e., Gamma rays, have dramatic effects on the structural and biological properties of DFDBA, leading to loss of BMPs. Ultraviolet-C radiation is a newer approach to sterilize biodegradable scaffolds, which is simple to use and ensures efficient sterilization. However, UV-C radiation has not yet been effectively studied to sterilize bone allografts. This study aimed to compare and evaluate the effectiveness of Gamma and Ultraviolet-C rays in sterilizing indigenously prepared DFDBA and assess their effect on the quantity of BMP-7 present in the allograft. DFDBA samples from non-irradiated, gamma irradiated, and UV-C irradiated groups were tested for BMP-7 level and samples sterilized with gamma and UV-C rays were analysed for sterility testing. The estimated mean BMP-7 level was highest in non-irradiated DFDBA samples, followed by UV-C irradiated, and the lowest in gamma irradiated samples. Our study concluded that UV-C rays effectively sterilized DFDBA as indicated by negative sterility test and comprised lesser degradation of BMP-7 than gamma irradiation.

脱矿冻干同种异体骨移植物（DFDBA）中骨形态发生蛋白的存在导致骨内缺损中硬组织的形成。同种异体骨移植最常见的灭菌方式，即伽马射线，对 DFDBA 的结构和生物学特性产生巨大影响，导致 BMP 损失。紫外线-C 辐射是一种较新的生物可降解支架灭菌方法，使用简单且可确保高效灭菌。然而，尚未有效研究 UV-C 辐射对同种异体骨移植物的灭菌作用。本研究旨在比较和评估伽马射线和紫外线 C 射线对本土制备的 DFDBA 进行灭菌的有效性，并评估它们对同种异体移植物中存在的 BMP-7 数量的影响。对来自未辐照组、伽马辐照组和 UV-C 辐照组的 DFDBA 样品进行 BMP-7 水平测试，并分析用伽马射线和 UV-C 射线灭菌的样品进行无菌测试。估计的平均 BMP-7 水平在未辐照的 DFDBA 样品中最高，其次是 UV-C 辐照的样品，在伽马辐照的样品中最低。我们的研究得出结论，如阴性无菌测试所示，UV-C 射线可以有效地对 DFDBA 进行灭菌，并且与伽马射线照射相比，BMP-7 的降解更少。