Abstract

Salmonella is a food-borne microorganism that is also a zoonotic bacterial hazard in the food sector. This study determined how well a mixed culture of Salmonella Kentucky formed biofilms on plastic (PLA), silicon rubber (SR), rubber gloves (RG), chicken skin and eggshell surfaces. In vitro interactions between the histone deacetylase inhibitor-vorinostat (SAHA)-and S. enterica serotype Kentucky were examined utilizing biofilms. The minimum inhibitory concentration (MIC) of SAHA was 120 µg mL⁻¹. The addition of sub-MIC (60 µg mL⁻¹) of SAHA decreased biofilm formation for 24 h on PLA, SR, RG, Chicken skin, and eggshell by 3.98, 3.84, 4.11, 2.86 and 3.01 log (p < 0.05), respectively. In addition, the initial rate of bacterial biofilm formation was higher on chicken skin than on other surfaces, but the inhibitory effect was reduced. Consistent with this conclusion, virulence genes expression (avrA, rpoS and hilA) and quorum-sensing (QS) gene (luxS) was considerably downregulated at sub-MIC of SAHA. SAHA has potential as an anti-biofilm agent against S. enterica serotype Kentucky biofilm, mostly by inhibiting virulence and quorum-sensing gene expression, proving the histone deacetylase inhibitor could be used to control food-borne biofilms in the food industry.

摘要

沙门氏菌是一种食源性微生物，也是食品领域的人畜共患细菌危害。这项研究确定了肯塔基州沙门氏菌混合培养物在塑料 (PLA)、硅橡胶 (SR)、橡胶手套 (RG)、鸡皮和蛋壳表面形成生物膜的效果。利用生物膜检查了组蛋白脱乙酰酶抑制剂伏立诺他 (SAHA) 和肯塔基州肠沙门氏菌血清型之间的体外相互作用。SAHA的最低抑菌浓度(MIC)为120μgmL ^-1。添加亚 MIC (60 µg mL ^-1 ) 的 SAHA 可将 PLA、SR、RG、鸡皮和蛋壳上的生物膜形成减少 3.98、3.84、4.11、2.86 和 3.01 log ( p  < 0.05)，持续 24 小时。分别。此外，鸡皮肤上细菌生物膜形成的初始速率高于其他表面，但抑制效果有所降低。与此结论一致，毒力基因表达（avrA、rpoS和hilA）和群体感应（QS）基因（luxS）在SAHA的亚MIC下显着下调。SAHA 有潜力作为针对肠沙门氏菌血清型肯塔基州生物膜的抗生物膜剂，主要通过抑制毒力和群体感应基因表达，证明组蛋白脱乙酰酶抑制剂可用于控制食品工业中的食源性生物膜。