In this study, we aimed to identify differentially expressed heat shock protein (HSP) profiles in the villi and decidua from patients with early missed abortion (EMA). By using high-throughput and high-precision parallel reaction monitoring (PRM)-based targeted proteomics techniques, this study examined the abundance of HSPs in the villi and decidua of 10 patients with EMA and 10 controls. Moreover, the abundance of 3 HSPs in the villi of another 22 patients with EMA and 22 controls was verified with Western blotting and immunohistochemistry (IHC). There were potential differences in the abundance of 16 HSPs and 42 polypeptides in human villi and decidua compared with those of the control group. Among them, HSP90AB1, HSPD1 and HSPA13 were downregulated in abundance in villi of patients with EMA, with a statistically significant difference, which was consistent with the verification results of Western blots and IHC. Using a PRM-based targeted proteomics technique, this study is the first to screen and quantitatively analyze the expression profile of HSPs in the villi and decidua of patients with EMA. The significant downregulation of HSP90AB1, HSPD1 and HSPA13 was found to have a potentially intimate association with the occurrence of EMA. The findings in our study may provide novel potential research targets related to HSPs for the pathogenesis, prevention and treatment of EMA.

中文翻译：

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基于 PRM 的定量蛋白质组学分析早期稽留流产患者绒毛和蜕膜中 HSP 丰度的变化

在这项研究中，我们的目的是鉴定早期稽留流产 (EMA) 患者绒毛和蜕膜中差异表达的热休克蛋白 (HSP) 谱。通过使用基于高通量和高精度平行反应监测（PRM）的靶向蛋白质组学技术，本研究检测了 10 名 EMA 患者和 10 名对照者的绒毛和蜕膜中 HSP 的丰度。此外，通过蛋白质印迹和免疫组织化学 (IHC) 验证了另外 22 名 EMA 患者和 22 名对照者的绒毛中 3 个 HSP 的丰度。人绒毛和蜕膜中16种HSP和42种多肽的丰度与对照组相比存在潜在差异。其中，HSP90AB1、HSPD1和HSPA13在EMA患者绒毛中大量下调，差异有统计学意义，与Western blots和IHC验证结果一致。本研究采用基于PRM的靶向蛋白质组学技术，首次筛选并定量分析EMA患者绒毛和蜕膜中HSP的表达谱。研究发现 HSP90AB1、HSPD1 和 HSPA13 的显着下调与 EMA 的发生具有潜在的密切相关性。我们的研究结果可能为 EMA 的发病机制、预防和治疗提供与 HSP 相关的新的潜在研究靶点。