In this study, the efficiency of two detectors—variable wavelength detector (VWD) and fluorescence detector (FLD)—in a liquid chromatograph to quantify azoxystrobin in water was compared. For this, a method was developed in a HPLC-VWD-FLD system using extraction by modified QuEChERS. The detection of azoxystrobin by fluorescence was possible using only a solvent effect, considering that azoxystrobin does not naturally fluoresce. Analytical curves were constructed using matrix-matched calibration, where a low matrix effect was verified in both detectors. In the linearity analysis of the analytical curves, the values of coefficient of determination ​​found for the detectors were 0.9989 and 0.9983 for VWD and FLD, respectively. The relative standard error for the VWD detector was 6.96%, while for the fluorescence detector, it was 5.49%. The limit of detection (LOD) and the limit of quantification (LOQ) were, respectively, 0.33 and 0.68 mg L⁻¹ (VWD) and 0.18 and 0.37 mg L⁻¹ (FLD). The QuEChERS method, originally developed for the extraction of pesticides in food, was adapted for the extraction of azoxystrobin in water, demonstrating efficiency in the process, with precision values ​​lower than 20% and accuracy between 83.58 and 112.88%, within the criteria established by MAPA and Document Nº SANTE/11312/2021. Therefore, it is concluded that both detectors are suitable for the analysis of azoxystrobin in water; however, there is a greater sensitivity for the fluorescence detector, since lower detection and quantification limit values ​​were observed, in addition to a lower standard relative error (RSE).

在本研究中，比较了液相色谱仪中两种检测器（可变波长检测器 (VWD) 和荧光检测器 (FLD)）对水中嘧菌酯定量的效率。为此，我们在 HPLC-VWD-FLD 系统中开发了一种使用改进的 QuEChERS 萃取的方法。考虑到嘧菌酯本身不发出荧光，仅利用溶剂效应就可以通过荧光检测嘧菌酯。使用基质匹配校准构建分析曲线，在两个探测器中验证了低基质效应。在分析曲线的线性分析中，发现VWD和FLD探测器的决定系数值分别为0.9989和0.9983。VWD检测器的相对标准误差为6.96%，而荧光检测器的相对标准误差为5.49%。⁻¹ (VWD) 以及 0.18 和 0.37 mg L ⁻¹ (FLD)。QuEChERS 方法最初是为食品中农药的提取而开发的，后来适用于水中嘧菌酯的提取，证明了该过程的效率，精度值低于 20%，准确度在 83.58 至 112.88% 之间，符合标准由 MAPA 和文件号 SANTE/11312/2021 建立。因此，两种检测器均适用于水中嘧菌酯的分析；然而，荧光检测器的灵敏度更高，因为除了较低的标准相对误差 (RSE) 之外，还观察到较低的检测和定量限值。