Abstract

The bacteriostatic and/or bactericidal properties of few phosphoramide-based amphiphilic compounds on human pathogenic bacteria were previously reported. In this study, the potential of two cationic (BSV36 and KLN47) and two zwitterionic (3 and 4) amphiphiles as inhibitors of marine bacterial growth and biofilm formation were investigated. Results showed that the four compounds have little impact on the growth of a panel of 18 selected marine bacteria at a concentration of 200 µM, and up to 700 µM for some bacterial strains. Interestingly, cationic lipid BSV36 and zwitterionic lipids 3 and 4 effectively disrupt biofilm formation of Paracoccus sp. 4M6 and Vibrio sp. D02 at 200 µM and to a lesser extent of seven other bacterial strains tested. Moreover, ecotoxicological assays on four species of microalgae highlighted that compounds 3 and 4 have little impact on microalgae growth with EC50 values of 51 µM for the more sensitive species and up to 200 µM for most of the others. Amphiphilic compounds, especially zwitterionic amphiphiles 3 and 4 seem to be promising candidates against biofilm formation by marine bacteria.

摘要

先前报道了少数基于磷酰胺的两亲性化合物对人类病原菌的抑菌和/或杀菌特性。在这项研究中，研究了两种阳离子（ BSV36和KLN47）和两种两性离子（3和4 ）两亲物作为海洋细菌生长和生物膜形成抑制剂的潜力。结果表明，这四种化合物在浓度为 200 µM 时对一组 18 种选定的海洋细菌的生长几乎没有影响，对于某些细菌菌株的浓度高达 700 µM。有趣的是，阳离子脂质BSV36和两性离子脂质3和4有效破坏副球菌生物膜的形成。4M6 和弧菌属。D02 为 200 µM，并在较小程度上测试了其他七种细菌菌株。此外，对四种微藻的生态毒理学测定强调，化合物3和4对微藻生长几乎没有影响，对于较敏感的物种，EC50 值为 51 µM，对于大多数其他物种，EC50 值为 200 µM。两亲化合物，尤其是两性离子两亲化合物3和4似乎是对抗海洋细菌生物膜形成的有希望的候选者。