Previously, we established that as a function of its mode of interaction with its ligand or cellular conditions such as membrane lipids, preexisting signaling intermediates activation status, a transmembrane receptor, as represented here with CD40, can induce counteractive cellular responses. Using CD40-binding peptides, recombinant mutated CD40-ligands, and an agonistic antibody, we have established the functional duality of CD40. CD40 builds up two constitutionally different signalosomes on lipid raft and non-raft membrane domains initiating two different signaling pathways. Although this initial signaling may be modified by the pre-existing signaling conditions downstream and may be subjected to feed-forward or negative signaling effects, the initial CD40-CD40L interaction plays a crucial role in the functional outcome of CD40. Herein, we have reviewed the influence of interaction between the CD40-CD40L evoking the functional duality of CD40 contingent upon different physiological states of the cells.

之前，我们确定，作为其与其配体或细胞条件（例如膜脂、预先存在的信号中间体激活状态）相互作用的模式，跨膜受体（如此处以 CD40 表示的）可以诱导相反的细胞反应。使用 CD40 结合肽、重组突变 CD40 配体和激动性抗体，我们建立了 CD40 的功能二元性。CD40 在脂筏和非筏膜域上构建两种结构不同的信号体，启动两种不同的信号传导途径。尽管这种初始信号传导可能会被下游预先存在的信号传导条件所修改，并且可能会受到前馈或负信号传导效应的影响，但初始的 CD40-CD40L 相互作用在 CD40 的功能结果中起着至关重要的作用。在此，我们回顾了 CD40-CD40L 之间相互作用的影响，引起 CD40 的功能二元性取决于细胞的不同生理状态。