Worldwide, fruit is an indispensable treasure house of nutrition for human beings, occupying a vital position of human diet. Postharvest fruit storage requires efficient antifungal agents to control Botrytis cinerea, which is a vital postharvest disease affecting fruit and leading to enormous losses. However, with the enormous abuse of existing antifungal drugs, the problem of drug-resistant fungi is imminent, making the controlling diseases caused by pathogenic fungi even more challenging. Drug repurposing is an efficient alternative method, we evaluated a well-known antifungal chemical, terbinafine, against the agricultural pathogen, B. cinerea in vitro, as a result, terbinafine showed strong antifungal activity. Furthermore, the in vivo antifungal activity of terbinafine was evaluated, the results showed that terbinafine could reduce the decay area on grapes. Terbinafine could disrupt the cell membrane integrity, increase cell membrane permeability, and eventual cell death of B. cinerea. In addition, terbinafine reduced decay incidence, and weight loss and maintained the soluble solids, titratable acidity, ascorbic acid, total phenolic, and malondialdehyde content during the storage period of grapes. Overall, terbinafine could be an antifungal preservative for postharvest table grapes fresh-keeping.

Graphical abstract

中文翻译：

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特比萘芬对采后葡萄抗灰霉病的质量保证

在世界范围内，水果是人类不可或缺的营养宝库，在人类饮食中占有重要地位。采后水果储存需要有效的抗真菌剂来控制灰葡萄孢，这是一种影响水果并导致巨大损失的重要采后病害。然而，随着现有抗真菌药物的大量滥用，耐药真菌问题迫在眉睫，使得病原真菌引起的疾病控制变得更加困难。药物再利用是一种有效的替代方法，我们在体外评估了一种著名的抗真菌化学物质特比萘芬对农业病原体灰霉病菌的作用，结果表明特比萘芬表现出很强的抗真菌活性。此外，还对特比萘芬的体内抗真菌活性进行了评价，结果表明特比萘芬可以减少葡萄的腐烂面积。特比萘芬会破坏细胞膜的完整性，增加细胞膜的通透性，最终导致灰霉病菌细胞死亡。此外，特比萘芬还降低了葡萄贮藏期间的腐烂发生率和重量损失，并保持了可溶性固形物、可滴定酸度、抗坏血酸、总酚和丙二醛含量。总体而言，特比萘芬可以作为采后鲜食葡萄保鲜的抗真菌防腐剂。

图形概要