Background: BSN-37, a novel antimicrobial peptide (AMP) containing 37 amino acid residues isolated from the bovine spleen, has not only antibacterial activity but also immunomodulatory activity. Recent evidence shows that long non-coding RNAs (lncRNAs) play an important role in regulating the activation and function of immune cells. The purpose of this experiment was to investigate the lncRNA and mRNA expression profile of mouse macrophages RAW264.7 stimulated by bovine antimicrobial peptide BSN-37. Methods: The whole gene expression microarray was used to detect the differentially expressed lncRNA and mRNA between antimicrobial peptide BSN-37 activated RAW264.7 cells and normal RAW264.7 cells. KEGG pathway analysis and GO function annotation analysis of differentially expressed lncRNAs and mRNA were carried out. Eight kinds of lncRNAs and nine kinds of mRNA with large differences were selected for qRT-PCR verification, respectively. Results: In the current study, we found that 1294 lncRNAs and 260 mRNAs were differentially expressed between antibacterial peptide BSN-37 treatment and control groups. Among them, Bcl2l12, Rab44, C1s, Cd101 and other genes were associated with immune responses and were all significantly up-regulated. Mest and Prkcz are related to cell growth, and other genes are related to glucose metabolism and lipid metabolism. In addition, some immune-related terms were also found in the GO and KEGG analyses. At the same time, real-time quantitative PCR was used to verify selected lncRNA and mRNA with differential expression. The results of qRT-PCR verification were consistent with the sequencing results, indicating that our data were reliable. Conclusion: This study provides the lncRNA and mRNA expression profiles of RAW264.7 macrophages stimulated by antimicrobial peptide BSN-37 and helps to provide a reference value for subsequent studies on lncRNA regulation of antimicrobial peptide BSN-37 immune function.

中文翻译：

---

抗菌肽BSN-37刺​​激巨噬细胞RAW264.7 lncRNA和mRNA表达谱综合分析

背景：BSN-37是从牛脾中分离得到的一种含有37个氨基酸残基的新型抗菌肽（AMP），不仅具有抗菌活性，还具有免疫调节活性。最近的证据表明，长链非编码RNA（lncRNA）在调节免疫细胞的激活和功能中发挥着重要作用。本实验的目的是研究牛抗菌肽BSN-37刺​​激小鼠巨噬细胞RAW264.7的lncRNA和mRNA表达谱。方法：采用全基因表达芯片检测抗菌肽BSN-37激活的RAW264.7细胞与正常RAW264.7细胞之间差异表达的lncRNA和mRNA。对差异表达的lncRNA和mRNA进行KEGG通路分析和GO功能注释分析。分别选取差异较大的8种lncRNA和9种mRNA进行qRT-PCR验证。结果：在本研究中，我们发现抗菌肽BSN-37治疗组和对照组之间有1294个lncRNA和260个mRNA存在差异表达。其中，Bcl2l12、Rab44、C1s、Cd101等基因与免疫反应相关，均显着上调。Mest和Prkcz与细胞生长有关，其他基因与糖代谢和脂质代谢有关。此外，GO和KEGG分析中还发现了一些与免疫相关的术语。同时采用实时定量PCR验证筛选出差异表达的lncRNA和mRNA。qRT-PCR验证结果与测序结果一致，说明我们的数据是可靠的。结论：本研究提供了抗菌肽BSN-37刺​​激RAW264.7巨噬细胞的lncRNA和mRNA表达谱，有助于为后续lncRNA调控抗菌肽BSN-37免疫功能的研究提供参考价值。