Experiments were conducted to optimize triploid induction parameters, and assess triploid sterility, in burbot. Duration and timing of shocks were based on degree minutes, temperature multiplied by time, denoted as °C min. Hydrostatic shock experiments investigated the duration of shock using 7500 or 8500 psi at 180°C min post-fertilization. Thermal shocks investigated duration of shock and post-fertilization shock timing using a shock of 16°C. Sterility experiments investigated egg survival when diploids were crossed with triploids. Hydrostatic shock of 7500 psi for 10 or 20°C min can induce triploidy ≥90% and exhibits survival that is statistically similar, p ≤ 0.05, to controls. Hydrostatic shock of 8500 psi for 5 or 10°C min yielded triploid induction of 93% and 100%, respectively, with survival that is statistically similar to controls, p ≤ 0.05. Thermal induction experiments indicated shocks at 120°C min post-fertilization, for durations between 350 and 450°C min, have potential to induce triploidy ≥90% while facilitating survival statistically similar to controls, p ≤ 0.05. Induction of tetraploidy was observed. Sterility experiments determined that triploid burbot are functionally sterile. These results may allow production of burbot where sterility is required.

中文翻译：

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三倍体江鳕，Lota lota，生产：热和静水参数的优化、四倍体诱导以及三倍体不育的确认

进行实验以优化江鳕三倍体诱导参数并评估三倍体不育性。冲击的持续时间和计时基于度/分钟、温度乘以时间，表示为°C 分钟。静水冲击实验研究了受精后在 180°C 分钟内使用 7500 或 8500 psi 的冲击持续时间。热冲击使用 16°C 的冲击研究冲击持续时间和受精后冲击时间。不育实验研究了二倍体与三倍体杂交时卵的存活率。7500 psi 的静水冲击持续 10 或 20°C 分钟可诱导三倍体 ≥90%，并且表现出与对照在统计学上相似的存活率 ( p  ≤ 0.05)。8500 psi 的静水冲击持续 5 或 10°C 分钟，三倍体诱导率分别为 93% 和 100%，存活率与对照在统计学上相似，p  ≤ 0.05。热感应实验表明，受精后 120°C 分钟的冲击，持续时间在 350 至 450°C 分钟之间，有可能诱导三倍体 ≥90%，同时促进存活，统计上与对照相似，p  ≤ 0.05。观察到四倍体的诱导。不育实验确定三倍体江鳕是功能性不育的。这些结果可能允许在需要无菌的情况下生产江鳕。