Abstract

Fibrin is formed via polymerization of one of the main blood proteins, fibrinogen, under the action of an enzyme, thrombin. Dynamic surface elasticity and dynamic surface tension of mixed solutions of fibrinogen and thrombin are measured as functions of surface age and enzyme concentration (50–800 U/L). The nonmonotonic pattern of the dependences for the dynamic surface elasticity indicates the multistage character of fibrin film formation and makes it possible to monitor the transition from unfolded protein to individual filamentous aggregates; a network of branched fibrils; and, finally, a continuous film. The dynamic surface elasticity of fibrin films is twofold higher than the corresponding values for fibrinogen (115 and 55 mN/m, respectively). The use of different types of microscopy makes it possible to assess the morphology of the obtained films.

中文翻译：

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纤维蛋白的动态表面特性

摘要

纤维蛋白是主要血液蛋白之一纤维蛋白原在凝血酶的作用下聚合形成的。纤维蛋白原和凝血酶混合溶液的动态表面弹性和动态表面张力作为表面年龄和酶浓度 (50-800 U/L) 的函数进行测量。动态表面弹性依赖性的非单调模式表明纤维蛋白膜形成的多阶段特征，并使监测从未折叠蛋白质到单个丝状聚集体的转变成为可能；分支原纤维网络；最后，一部连续的电影。纤维蛋白膜的动态表面弹性比纤维蛋白原的相应值高两倍（分别为 115 和 55 mN/m）。