Inhalation of nebulized TP has received little attention in the past. Here, we intend to investigate the effect of nebulized inhaled TP on airway inflammation in a mouse model of asthma. 29 SPF BALB/c mice were divided into four groups: blank control (Blk, n = 5), normal saline (NS, n = 8), dexamethasone (Dex, n = 8), and TP (n = 8). During the process of sensitization, mice in the three intervention groups were treated with nebulized NS, an injection of Dex, and nebulized triptolide, respectively. Then bronchoalveolar lavage fluid (BALF), peripheral blood, and lung tissue were collected. Relevant cytokines, transcriptional factors, and CD4+Th17+ T cell proportions were assessed and compared. IL-6, IL-17, IL-23, and TGF-β1 demonstrated a significant difference between groups in the following order: Dex < TP < NS (), while IL-10 changed in the opposite direction (). At the transcriptional level in lung tissue, the Ct value of IL-17 in the Dex group was significantly higher than in the NS and TP groups (). Meanwhile, it was higher in the TP group than in the NS group (). The Ct value of RORγt demonstrated a significant difference among three groups in the following order: Dex > TP > NS (). An opposite trend of FoxP3 Ct value was revealed in the order: NS > TP > Dex. The proportion of CD4+Th17+ cells was 9.53 ± 2.74% in the NS group, 4.23 ± 2.26% in the Dex group, and 6.76 ± 2.99% in the TP group, which shows significant differences between the NS and Dex () or NS and TP groups (). Inhalation of nebulized triptolide can play a role in suppressing airway inflammation with inflammatory cytokines and transcriptional factors reduced and CD4+Th17+ T cells dampened, also in a manner less than injected dexamethasone.

中文翻译：

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雾化吸入雷公藤甲素对哮喘小鼠模型气道炎症的影响

过去，雾化 TP 的吸入很少受到关注。在这里，我们打算研究雾化吸入TP对哮喘小鼠模型气道炎症的影响。29只SPF BALB/c小鼠分为四组：空白对照（Blk，n  = 5）、生理盐水（NS，n  = 8）、地塞米松（Dex，n  = 8）和TP（n  = 8）。致敏过程中，三个干预组分别给予雾化NS、注射Dex、雾化雷公藤甲素。然后收集支气管肺泡灌洗液（BALF）、外周血和肺组织。评估并比较相关细胞因子、转录因子和 CD4+Th17+ T 细胞比例。IL-6、IL-17、IL-23 和 TGF- β1按以下顺序显示组间显着差异：Dex < TP < NS（），而 IL-10 则朝相反方向变化（）。在肺组织转录水平上，Dex组IL-17的Ct值显着高于NS和TP组（）。同时，TP组高于NS组（）。ROR γ t的 Ct 值在三组之间表现出显着差异，顺序如下：Dex > TP > NS（）。FoxP3 Ct值呈现相反的趋势，顺序为：NS>TP>Dex。NS组CD4+Th17+细胞比例为9.53±2.74%，Dex组为4.23±2.26%，TP组为6.76±2.99%，显示NS​​和Dex之间存在显着差异（）或 NS 和 TP 组（）。雾化吸入雷公藤甲素可以抑制气道炎症，减少炎症细胞因子和转录因子，抑制 CD4+Th17+ T 细胞，其作用程度也低于注射地塞米松。