The first set of simple sequence repeat (SSR) markers for Urochloa mosambicensis (Hack.) Dandy is described in this study. SSRs were isolated from a genomic library with 3491 contigs obtained by high-throughput sequencing. Primers were designed for twenty loci, and all primer pairs were successfully amplified in 39 genotypes, yielding an average of 4.95 alleles per locus. The mean polymorphic information content (PIC) was 0.559, and the mean discriminating power (DP) was 0.520 for all loci considering also all U. mosambicensis genotypes. The non‐Bayesian clustering analysis has confirmed clustering consistency of the individuals and provides enough support for the existence of the pattern that also emerged from PCoA and UPGMA. These markers demonstrated potential transferability to U. advena, U. oligotricha, U. brachyura and U. xantholeuca. Our findings showed that the set of molecular markers described here have strong potential for fingerprinting and characterizing genetic diversity in intra-specific plant collections, whose breeding programs could be accelerated by the effective use of these molecular tools, not only in U. mosambicensis, but also within the genus Urochloa.

本研究描述了Urochloa mosambicensis (Hack.) Dandy的第一组简单序列重复 (SSR) 标记。SSR 是从基因组文库中分离出来的，该文库包含通过高通量测序获得的 3491 个重叠群。为20个位点设计了引物，所有引物对均在39个基因型中成功扩增，每个位点平均产生4.95个等位基因。所有位点的平均多态信息含量 (PIC) 为 0.559，平均判别力 (DP) 为 0.520，还考虑到所有莫桑比克U. mosambicensis基因型。非贝叶斯聚类分析证实了个体的聚类一致性，并为 PCoA 和 UPGMA 中出现的模式的存在提供了足够的支持。这些标记证明了对U. advena、U.oligotricha、U.brachyura和U.xantholeuca的潜在可转移性。我们的研究结果表明，这里描述的这组分子标记具有强大的潜力，可以用于指纹识别和表征特定种内植物集合中的遗传多样性，通过有效使用这些分子工具可以加速其育种计划，不仅在莫桑比克 U. mosambicensis 中，而且在 U. mosambicensis中。也在尾草属内。