Abstract

Objective

Circadian rhythmicity has been shown to contribute to the regulation of key physiological and cognitive processes related to performance. The period homolog 3 (PER3) is expressed in a circadian pattern in the suprachiasmatic nucleus. Therefore, in this study, we aimed to evaluate the role of the variable tandem repeat (VNTR) variant of the PER3 gene in athletic performance in the Turkish population.

Methods

This study included 223 subjects, which consisted of 123 athletes and 100 sedentary controls. Blood samples were drawn from all subjects. DNA was extracted from whole-blood samples. The PER3 VNTR variant was genotyped using the polymerase chain reaction-restriction method (PCR). The results of the analyses were evaluated for statistical significance.

Results

The mean ages of athletes and controls were 22 ± 2.814 and 23 ± 3.561, respectively. Endurance athletes in the group were 21.1%, and sprint athletes were 78.9%. There was no statistical significance in terms of PER3 VNTR genotype distribution or allele frequency. In the recessive model, a statistically significant association was observed when the athletes were compared with the controls according to 4/4 + 4/5 versus 5/5 genotype (p = 0.020).

Conclusion

In this case-control study, for the first time in our country, we obtained findings suggesting that the PER3 VNTR variant may affect sports performance in the Turkish population. Results need to be replicated in different ethnic and larger samples.

中文翻译：

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土耳其运动员昼夜节律基因 (PER3) VNTR 变异的评估

摘要

客观的

昼夜节律已被证明有助于调节与表现相关的关键生理和认知过程。周期同源物 3 ( PER3 ) 在视交叉上核中以昼夜节律模式表达。因此，在这项研究中，我们旨在评估PER3基因的可变串联重复序列 (VNTR) 变体在土耳其人群运动表现中的作用。

方法

这项研究包括 223 名受试者，其中包括 123 名运动员和 100 名久坐的对照组。从所有受试者身上抽取血液样本。从全血样本中提取 DNA。使用聚合酶链式反应限制法 (PCR) 对PER3 VNTR变体进行基因分型。评估分析结果的统计显着性。

结果

运动员和对照组的平均年龄分别为 22 ± 2.814 岁和 23 ± 3.561 岁。该组中耐力运动员为21.1%，短跑运动员为78.9%。PER3 VNTR 基因型分布或等位基因频率没有统计学意义。在隐性模型中，当根据 4/4 + 4/5 与 5/5 基因型（ p  = 0.020）将运动员与对照组进行比较时，观察到统计学上显着的关联。

结论

在这项病例对照研究中，我们在我国首次获得了表明PER3 VNTR 变体可能影响土耳其人群运动表现的研究结果。结果需要在不同种族和更大的样本中重复。