Abstract

Aberrant activation of Wnt pathway is linked to dysregulation of several genes. DACT1 and DACT2 are members of the DACT family that participate in antagonizing of the Wnt signaling cascade. Thus in this study, we assessed the mRNA levels of DACT1, DACT2, and CYCLIN D1 in 70 pairs of CRC tissues compared to the adjacent tissues. Determination of the mRNA levels of DACT1, DACT2, and CYCLIN D1 was done by Quantitative Real-Time PCR (qRT-PCR). The correlation between DACT1, DACT2, and CYCLIN D1 genes was also examined. Receiver operating characteristic (ROC) curves was plotted to assess the diagnostic power. The association between histopathological parameters and the DACT1, DACT2, and CYCLIN D1 genes was investigated. The expression levels of DACT1 and CYCLIN D1 were remarkably higher in CRC tissues compared to the adjacent tissues (p < 0.0001). However, the expression of DACT2 was decreased (p < 0.001). Our results showed a significant correlation between the expression of DACT1 and CYCLIN D1 (p < 0.0001). DACT1 (AUC = 0.74, p < 0.0001), DACT2 (AUC = 0.69, p < 0.0003), and CYCLIN D1 (AUC = 0.75, p < 0.0001) had good effectiveness in separation between CRC samples and adjacent tissues. We found a significant association between DACT1 expression with tumor site (p < 0.01). Also, a significant association was detected between DACT2 and CYCLIN D1 with tumor stage (p < 0.005 and p < 0.038, respectively). The findings suggested that DACT1 could function as an oncogene, whereas DACT2 was downregulated and can be considered as a tumor suppressor in CRC.

摘要

Wnt 通路的异常激活与多个基因的失调有关。DACT1 和 DACT2 是 DACT 家族的成员，参与 Wnt 信号级联的拮抗。因此，在本研究中，我们评估了70 对 CRC 组织中与邻近组织相比的DACT1、DACT2和CYCLIN D1的 mRNA 水平。通过定量实时PCR (qRT-PCR)测定DACT1、DACT2和CYCLIN D1的mRNA水平。还检查了DACT1、DACT2和CYCLIN D1基因之间的相关性。绘制受试者工作特征（ROC）曲线以评估诊断能力。研究了组织病理学参数与DACT1、DACT2和CYCLIN D1基因之间的关联。与癌旁组织相比，CRC 组织中DACT1和CYCLIN D1的表达水平显着较高 ( p  < 0.0001)。然而， DACT2的表达降低 ( p  < 0.001)。我们的结果显示DACT1和CYCLIN D1的表达之间存在显着相关性( p  < 0.0001)。DACT1（AUC = 0.74，p  < 0.0001）、DACT2（AUC = 0.69，p  < 0.0003）和CYCLIN D1（AUC = 0.75，p  < 0.0001）在分离 CRC 样本和邻近组织方面具有良好的效果。我们发现DACT1表达与肿瘤部位之间存在显着相关性( p  < 0.01)。此外，还检测到DACT2和CYCLIN D1与肿瘤分期之间存在显着相关性（分别为p < 0.005和p  < 0.038）。研究结果表明，DACT1可以作为癌基因发挥作用，而DACT2则下调，可以被视为 CRC 中的肿瘤抑制基因。