Biodegradability and cellular activity are key performance indicators that should be prioritized for tissue engineering applications. Biopolymer selection, determination of necessary structural properties, and their synergistic interactions play an active role in obtaining the expected biodegradability and biological activity from scaffolds. In this study, it is aimed to produce electrospun webs with improved biocompatibility by blending polycaprolactone (PCL) with polylactic acid (PLA) and poly-l-lactide (PLLA), and examine the effect of biopolymer selection and blend ratio on the biodegradability and cellular activity of surfaces. In this context, fibrous webs are produced from PCL/PLA and PCL/PLLA blends with a weight ratio of 80/20 and 50/50 and pure polymers of PCL, PLA, and PLLA by electrospinning method and subjected to morphological and biological analyses. The biodegradation tests are carried out hydrolytically while the cell viability and cell proliferation analyses are performed with adult human primary dermal fibroblasts and human umbilical endothelial cells (HUVECs). The results show that the fiber diameters of the fabricated webs ranged from 0.747 to 1.685 μm. At the end of the 5th month, it is observed that the biodegradation rates of the webs blended 50% with PLA and PLLA, in comparison to PCL ones, increase from 3.7% to 13.33% and 7.69%, respectively. On the other hand, cell culture results highlight that the addition of 20% PLA and PLLA improves the cellular activity of both cell types, but increased PLA or PLLA ratio in PCL webs has a negative effect as it makes the structure stiff and brittle.

中文翻译：

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研究用于组织工程应用的 PCL/PLA 和 PCL/PLLA 电纺网的生物降解性和细胞活性

生物降解性和细胞活性是组织工程应用应优先考虑的关键性能指标。生物聚合物的选择、必要结构特性的确定及其协同相互作用在支架获得预期的生物降解性和生物活性方面发挥着积极作用。在本研究中，旨在通过将聚己内酯（PCL）与聚乳酸（PLA）和聚L-丙交酯（PLLA）共混来生产具有改善的生物相容性的静电纺丝网，并研究生物聚合物的选择和共混比例对生物降解性和生物相容性的影响。表面的细胞活性。在这种情况下，通过静电纺丝方法由重量比为 80/20 和 50/50 的 PCL/PLA 和 PCL/PLLA 共混物以及 PCL、PLA 和 PLLA 的纯聚合物生产纤维网，并进行形态学和生物学分析。生物降解测试通过水解进行，而细胞活力和细胞增殖分析则使用成人原代真皮成纤维细胞和人脐带内皮细胞 (HUVEC) 进行。结果表明，所制造的纤维网的纤维直径范围为0.747至1.685μm。在第5个月末，观察到与PCL共混50%的纤维网的生物降解率分别从3.7%增加到13.33%和7.69%。另一方面，细胞培养结果强调，添加 20% PLA 和 PLLA 可以提高两种细胞类型的细胞活性，但 PCL 网中 PLA 或 PLLA 比例的增加会产生负面影响，因为它会使结构僵硬且易碎。