Zn²⁺ is involved in various physiological and pathological processes in living systems. Monitoring the dynamic spatiotemporal changes of Zn²⁺ levels in organelles, cells, and in vivo is of great importance for the investigation of the physiological and pathological functions of Zn²⁺. However, this task is quite challenging since Zn²⁺ in living systems is present at low concentrations and undergoes rapid dynamic changes. In this review, we summarize the design and application of fluorescent probes for Zn²⁺ imaging in organelles, cells, and live organisms reported over the past two years. We aim to provide inspiration for the design of novel Zn²⁺ probes for multi-level monitoring and deepen the understanding of Zn²⁺ biology.

Zn ²⁺参与生命系统的各种生理和病理过程。^{监测细胞器、细胞和体内Zn 2+}水平的动态时空变化对于研究Zn ²⁺的生理和病理功能具有重要意义。然而，这项任务非常具有挑战性，因为生命系统中的 Zn ²⁺浓度较低并且经历快速的动态变化。在这篇综述中，我们总结了过去两年报道的细胞器、细胞和活体中Zn ²⁺成像荧光探针的设计和应用。^{我们的目的是为多层次监测的新型Zn 2+}探针的设计提供灵感，并加深对Zn ²⁺生物学的理解。