Abstract

Dillenia indica is a medicinal plant used in the indigenous system of medicine for plethora of diseases. In this study, the efficacy of ethanolic bark extract of Dillenia indica was investigated for green synthesis of copper nanoparticles (CuNPs). Obtained CuNPs were crystalline, spherical in shape within a size range of 20.87–45.73 nm, well dispersed showing strong negative zeta potential value (−41.8 mV) as analysed by XRD, TEM, FESEM and particle size analyser. The elemental composition of CuNPs was examined through EDX spectrum followed by field map analysis. FTIR spectra showed the presence of active biomolecules responsible for reduction of copper ions as well as for surface functionalisation of CuNPs. Synthesised CuNPs showed marked free radical scavenging efficacy at IC50 of 37.2 µg/mL. MTT assay in CuNPs treated A549 and MCF-7 cancer cell lines for 24 and 48 h showed substantial decrease of cell viability with increase in concentration. Further, morphological alterations like cell shrinkage, nuclear fragmentation and blebbing were remarkably observed in CuNPs treated both the cell lines as assayed through AO/EtBr and DAPI staining methods. Thus, D. indica bark extract-mediated CuNPs were potential free radical scavengers and effective cytotoxic against A549 and MCF-7 cancer cell lines.

摘要

Dillenia indica是一种药用植物，在本土医学系统中用于治疗多种疾病。在本研究中， Dillenia indica的乙醇树皮提取物的功效研究了铜纳米颗粒（CuNP）的绿色合成。所得 CuNPs 呈结晶状，呈球形，尺寸范围为 20.87–45.73 nm，分散良好，通过 XRD、TEM、FESEM 和粒度分析仪分析显示出强负 zeta 电位值（-41.8 mV）。通过 EDX 光谱和场图分析检查了 CuNP 的元素组成。FTIR 光谱显示活性生物分子的存在负责铜离子的还原以及 CuNP 的表面功能化。合成的 CuNPs 显示出显着的自由基清除功效，IC50 为 37.2 µg/mL。CuNPs 处理 A549 和 MCF-7 癌细胞系 24 和 48 小时的 MTT 分析显示，随着浓度的增加，细胞活力显着下降。此外，细胞收缩等形态变化，通过 AO/EtBr 和 DAPI 染色方法测定，在 CuNP 处理的两种细胞系中均明显观察到核碎片和起泡。因此，D. indica树皮提取物介导的 CuNP 是潜在的自由基清除剂，对 A549 和 MCF-7 癌细胞系具有有效的细胞毒性。