This study was designed to characterize mice bone marrow (BM) and bone marrow-derived dendritic cells (BMDC) and to compare the surface markers expression and inflammatory cytokine liberation in response to LPS and Bothrops jararacussu venom (BjV) stimulation. Typical morphology was observed in BM and BMDCs from the 4th up to the 8th day of culture using recombinant mouse GM-CSF and IL-4. A high basal level of MHC-II, CD1d, CD83, CD11c, CD80, and low CD86 was expressed by BM cells. After stimulation with GM-CSF/IL-4 for BMDCs differentiation, the BM cells differentiated into BMDCs presented MHC-II, CD1d, CD83, CD11c, CD86, and CD80 expression on the 4th – 8th day accompanied with high levels of TNF-α liberated. The difference between the surface markers' expression was observed in this time course in which CD1d, CD11c, and CD80 remained in high levels of expression, while MHC-II and CD83 showed moderate expression during the differentiation period. Also, cytokines liberation was monitored over the period of the BMDCs culture, and on the 6th day, low levels of IL-6 and IL-1β were found, while high levels of TNF-α on the 4th and 8th days, both of which contributed to the maturity of the BMDCs. Maturation of DCs with LPS showed significant upregulation of surface markers (MHC-II, CD1d, CD83, CD86, CD80) and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) liberation. On the other hand, BjV induced a decrease in CD1d, CD11c, CD83, and CD86 expression in mature BMDCs which was not observed when LPS was used to stimulate BMDCs which probably induces impairment in T-cell activation.

本研究旨在表征小鼠骨髓 (BM) 和骨髓源性树突状细胞 (BMDC)，并比较 LPS 和白腹蛇毒液 (BjV) 刺激后的表面标志物表达和炎症细胞因子释放。使用重组小鼠 GM-CSF 和 IL-4，从培养第 4 天到第 8 天，在 BM 和 BMDC 中观察到典型的形态。BM细胞表达高基础水平的MHC-II、CD1d、CD83、CD11c、CD80和低水平的CD86。GM-CSF/IL-4刺激BMDC分化后，分化为BMDC的BM细胞在第4-8天出现MHC-II、CD1d、CD83、CD11c、CD86和CD80表达，并伴有高水平的TNF-α解放了。在此时间过程中观察到表面标记表达的差异，其中CD1d、CD11c和CD80保持高水平表达，而MHC-II和CD83在分化期间表现出中等表达。此外，在 BMDC 培养期间监测细胞因子释放，在第 6 天发现低水平的 IL-6 和 IL-1β，而在第 4 天和第 8 天发现高水平的 TNF-α。有助于 BMDC 的成熟。使用 LPS 使 DC 成熟后，表面标记物（MHC-II、CD1d、CD83、CD86、CD80）和促炎细胞因子（IL-1β、IL-6、TNF-α）释放显着上调。另一方面，BjV 诱导成熟 BMDC 中 CD1d、CD11c、CD83 和 CD86 表达的减少，而当使用 LPS 刺激 BMDC 时未观察到这一点，这可能会导致 T 细胞活化受损。