Soil contamination with micropollutants is an important global problem and the impact of these pollutants on living organisms cannot be underestimated. The effects of diclofenac (DCF) and sulfamethoxazole (SMX), their mixture (MIX), and wastewater containing these drugs on the mortality and reproduction of Eisenia fetida were investigated. The impact on the activities of antioxidant enzymes in earthworm cells was also assessed. Furthermore, the influence of the following parameters of the vertical flow constructed wetlands on wastewater toxicity was investigated: the dosing system, the presence of pharmaceuticals and the plants Miscanthus giganteus. The compounds and their mixture significantly affected the reproduction and mortality of earthworms. The calculated values of LC_{50,28 days} values were 3.4 ± 0.3 mg kg⁻¹ for DCF, 1.6 ± 0.3 mg kg⁻¹ for SMX, and 0.9 ± 0.1 mg kg⁻¹ for MIX. The EC₅₀ (reproduction assay) for DCF was 1.2 ± 0.2 mg kg⁻¹, whereas for SMX, it was 0.4 ± 0.1 mg kg⁻¹, and for MIX, it was 0.3 ± 0.1 mg kg⁻¹, respectively. The mixture toxicity index (MTI) was calculated to determine drug interactions. For both E. fetida mortality (MTI = 3.29) and reproduction (MTI = 3.41), the index was greater than 1, suggesting a synergistic effect of the mixture. We also observed a negative effect of wastewater (raw and treated) on mortality (32% for raw and 8% for treated wastewater) and fertility (66% and 39%, respectively) of E. fetida. It is extremely important to analyze the harmfulness of microcontaminants to organisms inhabiting natural environments, especially in the case of wastewater for irrigation of agricultural fields.

土壤微污染物污染是一个重要的全球性问题，这些污染物对生物体的影响不可低估。研究了双氯芬酸 (DCF) 和磺胺甲恶唑 (SMX)、它们的混合物 (MIX) 以及含有这些药物的废水对赤子爱胜蚓 ( Eisenia fetida ) 死亡率和繁殖的影响。还评估了对蚯蚓细胞中抗氧化酶活性的影响。此外，还研究了垂直流人工湿地的以下参数对废水毒性的影响：加药系统、药物的存在和植物芒。这些化合物及其混合物显着影响蚯蚓的繁殖和死亡率。_{LC 50.28 天}的计算值对于 DCF为 3.4 ± 0.3 mg kg ^-1 ，对于 SMX 为1.6 ± 0.3 mg kg ^-1 ，对于 MIX 为0.9 ± 0.1 mg kg ^{-1 。}DCF的EC ₅₀（繁殖测定）为1.2±0.2mg kg ^-1，而SMX的EC 50 为0.4±0.1mg kg ^-1，MIX的EC 50 为0.3±0.1mg kg ^-1。计算混合物毒性指数（MTI）以确定药物相互作用。对于臭臭艾美奖死亡率 (MTI = 3.29) 和繁殖 (MTI = 3.41)，该指数都大于 1，表明混合物具有协同效应。我们还观察到废水（未经处理的废水和经过处理的废水）对臭艾美蛔虫的死亡率（未经处理的废水为 32%，经过处理的废水为 8%）和繁殖力（分别为 66% 和 39%）产生负面影响。分析微污染物对自然环境中生物体的危害极其重要，特别是农田灌溉废水。