Water deficit is one of the most important climate events that has strong effect on agricultural ecosystem functionality comprising soil microbial communities and their functions. Arbuscular mycorrhizal fungi (AMF) are widely known for their roles in combating drought, including facilitation of drought-tolerant bacteria. However, differences in cultivar/variety affinity for mycorrhization have never been considered as influencing factors. In the present study, we evaluated the influence of mycorrhizal affinity of two durum wheat (T. turgidum subsp. Durum (Desf.)) varieties, Iride and Ramirez (high and low, respectively) on root and soil bacteriomes under well-watered and drought conditions. We used the 16S metagenomics approach (amplicon sequencing) to assess the bacterial communities of root and soil samples. The suppression effect of drought was evident across a wide range of bacterial taxa, including drought-tolerant taxa, especially in the non-inoculated plants. Nevertheless, the protective effect of AMF was also shown, especially in the Iride variety (high AMF colonization affinity) in both compartments (root and soil), as the relative abundance of drought-depleted taxa, such as Planctomycetes, Bacteroidetes and Verrucomicrobia, was either similar under well-watered and water deficit conditions or increased under water deficit conditions. Moreover, drought reduced the network complexity of root and soil bacteria, especially in Ramirez variety which has a lower AMF colonization affinity. Together, our results suggest that not only AMF colonization, but also host plant colonization affinity is one of the regulating factors in alleviating drought-induced changes in wheat plants by altering plant-fungal-bacterial interactions.

缺水是最重要的气候事件之一，对农业生态系统功能（包括土壤微生物群落及其功能）有强烈影响。丛枝菌根真菌（AMF）因其在抗旱方面的作用而广为人知，包括促进耐旱细菌的生长。然而，品种/品种对菌根化亲和力的差异从未被视为影响因素。在本研究中，我们评估了两种硬粒小麦（T. turgidum subsp. Durum (Desf.)）品种 Iride 和 Ramirez（分别为高和低）的菌根亲和力对浇水良好和土壤细菌组的影响。干旱条件。我们使用 16S 宏基因组学方法（扩增子测序）来评估根和土壤样本的细菌群落。干旱的抑制作用在多种细菌类群中都很明显，包括耐旱类群，尤其是在未接种的植物中。然而，AMF 的保护作用也得到了体现，特别是在 Iride 品种（高 AMF 定殖亲和力）中，两个区室（根和土壤）中，由于干旱耗竭的类群（如浮霉菌、拟杆菌和疣微菌）的相对丰度降低，因此 AMF 的保护作用也得到了体现。或者在浇水良好和缺水条件下相似，或者在缺水条件下增加。此外，干旱降低了根部和土壤细菌的网络复杂性，尤其是在 AMF 定植亲和力较低的 Ramirez 品种中。总之，我们的结果表明，不仅 AMF 定植，