Cytokine-induced killer (CIK) cells have shown promising results in adoptive immunotherapy. However, serum may play a determining role in the large-scale expansion of these cells for clinical applications. According to Good Manufacturing Practice (GMP) guidelines to reduce the use of animal products in cell-based therapies; therefore, this study sought to investigate the impact of serum origin and the reduced serum concentration on the pattern of cell expansion and function. Peripheral blood mononuclear cells (PBMCs) isolated from a healthy donor were expanded based on the CIK cell expansion protocol. The cell culture medium was supplemented with three types of sera comprising fetal bovine serum (FBS), human serum (HS), or human-derived platelet lysate (hPL) at different concentrations (10%, 5%, and 2.5%). The proliferation kinetics for each group were investigated for 30 days of cell culture. Cell proliferation in 10% concentration of all sera (hPL, FBS, HS) was higher than their lower concentrations. Moreover, hPL was significantly associated with higher expansion rates than FBS and HS in all three concentrations. Furthermore, cells cultured in hPL showed higher viability, cytotoxicity effect, and CIK CD markers expression. hPL at a concentration of 10% showed the best effect on CIK cell proliferation and function.

中文翻译：

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血清来源对细胞因子诱导的杀伤细胞扩增和功能的影响

细胞因子诱导的杀伤（CIK）细胞在过继免疫疗法中显示出有希望的结果。然而，血清可能在这些细胞大规模扩增用于临床应用中发挥决定性作用。根据良好生产规范（GMP）指南，减少细胞疗法中动物产品的使用；因此，本研究旨在研究血清来源和血清浓度降低对细胞扩增和功能模式的影响。根据 CIK 细胞扩增方案扩增从健康供体分离的外周血单核细胞 (PBMC)。细胞培养基补充三种类型的血清，包括不同浓度（10%、5%和2.5%）的胎牛血清（FBS）、人血清（HS）或人源血小板裂解物（hPL）。研究细胞培养 30 天后各组的增殖动力学。所有血清（hPL、FBS、HS）浓度为 10% 时的细胞增殖均高于其较低浓度。此外，在所有三个浓度中，hPL 与比 FBS 和 HS 更高的扩增率显着相关。此外，在 hPL 中培养的细胞表现出更高的活力、细胞毒性作用和 CIK CD 标记物表达。10%浓度的hPL对CIK细胞增殖和功能表现出最佳效果。