Cervical cancer is a common malignancy among women worldwide. Long non-coding RNAs (lncRNAs) are frequently involved in the pathogenesis of cervical cancer. Therefore, the present study aimed to investigate the potentials of lncRNA799 in cervical cancer. mRNA and protein expression were detected by reverse transcription-quantitative polymerase chain reaction and Western blot analysis, respectively. Cellular functions were assessed using CCK-8, wound healing and transwell analysis. The binding potential of zinc finger E-box-binding homeobox 1 (ZEB1) on the promoter of lncRNA799 was predicted utilizing the JASPAR database, and was then verified by luciferase and chromatin immunoprecipitation (ChIP) assays. Furthermore, the gene interactions were assessed using RNA immunoprecipitation and co-immunoprecipitation assays. The results demonstrated that lncRNA799 was upregulated in cervical cancer cells. However, lncRNA799 deficiency suppressed the proliferation and epithelial-mesenchymal transition of cervical cancer cells. Furthermore, lncRNA799 could interact with eukaryotic translation initiation factor 4A3 to maintain the mRNA stability of transducin (β)-like 1 X-linked receptor 1 (TBL1XR1) and promote the interaction between ZEB1 and TBL1XR1. Additionally, the results showed that ZEB1 could transcriptionally activate lncRNA799. Taken together, the present study suggested that the lncRNA799/TBL1XR1/ZEB1 axis could form a positive feedback loop in cervical cancer and could be, therefore, considered as a potential therapeutic strategy for cervical cancer.

中文翻译：

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lncRNA799/TBL1XR1/ZEB1轴形成反馈环促进宫颈癌细胞上皮-间质转化

宫颈癌是全世界女性常见的恶性肿瘤。长链非编码RNA（lncRNA）经常参与宫颈癌的发病机制。因此，本研究旨在探讨lncRNA799在宫颈癌中的潜力。分别通过逆转录定量聚合酶链反应和蛋白质印迹分析检测mRNA和蛋白表达。使用 CCK-8、伤口愈合和 Transwell 分析评估细胞功能。利用 JASPAR 数据库预测锌指 E 盒结合同源盒 1 (ZEB1) 在 lncRNA799 启动子上的结合潜力，然后通过荧光素酶和染色质免疫沉淀 (ChIP) 测定进行验证。此外，使用 RNA 免疫沉淀和免疫共沉淀测定评估基因相互作用。结果表明lncRNA799在宫颈癌细胞中表达上调。然而，lncRNA799缺陷抑制了宫颈癌细胞的增殖和上皮间质转化。此外，lncRNA799可以与真核翻译起始因子4A3相互作用，维持转导蛋白（β）样1 X连锁受体1（TBL1XR1）的mRNA稳定性，并促进ZEB1和TBL1XR1之间的相互作用。此外，结果表明ZEB1可以转录激活lncRNA799。综上所述，本研究表明lncRNA799/TBL1XR1/ZEB1轴可以在宫颈癌中形成正反馈环，因此可以被视为宫颈癌的潜在治疗策略。