Abstract

Introduction

Pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity, and relationship has been determined between antibiotic resistance and biofilm. The aim of this study was to investigate the virulence factors of Pseudomonas aeruginosa isolates by genotypic and phenotypic methods, as well as whether there is a relationship between other virulence factors and antibiotic resistance.

Methods

A total of 80 Pseudomonas aeruginosa strains were sent from various clinics included in the study. Identification and antibiotic resistance profile of isolates were determined by Vitek 2 (Biomerioux, France) automated system. Pseudomonas P agar, Pseudomonas F agar, and motility test medium were used for phenotyping tests. Tox A, Exo S, Plc N, and Las B were evaluated with Real-time PCR (Anatolia, Geneworks, Turkey).

Results

The highest rates of antibiotic resistance were observed against imipenem (42.5%) and meropenem (40%). Among the isolates, 81.3% tested positive for Tox A, 30% for Exo S, 32.5% for Plc N, and 42.5% for Las B. Additionally, 70.4% of the isolates tested positive for pyocyanin, 41.3% for pyoverdine, 1.8% for pyorubin, and 8.9% tested negative for pyorubin. No statistically significant difference was found between antibiotic resistance and the presence of virulence factors (p > 0.005).

Conclusions

The relationship between antibiotic resistance and virulence factors is controversial. There are studies demonstrating the relationship between virulence factors and antibiotic resistance, as well as studies that indicate the absence of such a relationship. Investigating virulence and antibiotic resistance rates may be important for identifying potential drug targets for subsequent research.

中文翻译：

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表型和基因型方法研究铜绿假单胞菌毒力因子

摘要

介绍

铜绿假单胞菌拥有多种可能导致其致病性的毒力因子，并且抗生素耐药性与生物膜之间的关系已确定。本研究的目的是通过基因型和表型方法探讨铜绿假单胞菌分离株的毒力因子，以及其他毒力因子与抗生素耐药性之间是否存在关系。

方法

研究中的各个诊所总共送出了 80 株铜绿假单胞菌菌株。分离株的鉴定和抗生素耐药性谱由 Vitek 2（Biomerioux，法国）自动化系统确定。使用假单胞菌P 琼脂、假单胞菌F 琼脂和运动测试培养基进行表型测试。使用实时 PCR（Anatolia、Geneworks、土耳其）评估Tox A、Exo S、Plc N和Las B。

结果

亚胺培南 (42.5%) 和美罗培南 (40%) 的抗生素耐药率最高。在分离株中，81.3% 的Tox A检测呈阳性，30% 的Exo S检测呈阳性，32.5% 的Plc N检测呈阳性，42.5% 的Las B检测呈阳性。此外，70.4% 的分离株绿脓菌素检测呈阳性，41.3% 的菌素检测呈阳性，1.8% 的分离株绿红素检测呈阳性，8.9% 的分离株绿脓素检测呈阴性。抗生素耐药性和毒力因子的存在之间没有发现统计学上的显着差异（p  > 0.005）。

结论

抗生素耐药性与毒力因子之间的关系存在争议。有研究证明毒力因子与抗生素耐药性之间的关系，也有研究表明不存在这种关系。研究毒力和抗生素耐药率对于确定后续研究的潜在药物靶点可能很重要。