In vitro anther and ovule culture has been mostly used in haploidization studies of annual and perennial plants to shorten the process of breeding. Cyclamen genus is one of the major perennial geophytes widely used as an ornamental plant. The aim of this study was to develop an efficient haploid plant regeneration protocol via anther and ovule culture for wild Cyclamen persicum and commercial F1 Melody cultivar. The uninuclear stage of microspore was determined with DAPI (4′,6-diamidino-2-phenylindole dihydrochloride) dye for C. persicum Mill. and commercial cultivar. Cold pre-treatment (4 °C) was applied to the buds for two d before in vitro ovule and anther cultures. Anthers were cultured on B5 medium combined with different dosages of 1-naphthaleneacetic acid (NAA), 135.0 g L⁻¹ maltose, silver nitrate (AgNO₃), and activated charcoal (AC). Ovules were cultured on Murashige and Skoog (MS) medium including the varied amount of 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-(2-isopentenyladenine) (2iP) and sucrose. Embryos were maturated and germinated on the M2 medium (MS containing 0.2 mg L⁻¹ giberellic acid (GA₃), 0.1 mg L⁻¹ 6-benzylaminopurine (BA), 1.0 g L⁻¹ proline, and 0.05 mg L⁻¹ spermine) for anther culture and MS medium without plant growth regulator (PGR) for ovule culture. Haploid embryos were obtained from B5 medium, including 1.0 mg L⁻¹ NAA for C. persicum Mill. (100%). An efficient ovule culture protocol was determined for C. persicum Mill. as 2.0 mg L⁻¹ 2,4-D and 0.8 mg L⁻¹ 2iP; and 2.0 mg L⁻¹ 2,4-D and 0.5 mg L⁻¹ 2iP for Melody F1 cultivar as 100%. Spontaneous double haploidization was detected on C. persicum Mill. via flow cytometric analysis. Plants were transferred to the soil, and blooming was observed 4 mo after acclimatization.

体外花药和胚珠培养主要用于一年生和多年生植物的单倍化研究，以缩短育种过程。仙客来属是广泛用作观赏植物的主要多年生地球植物之一。本研究的目的是通过野生仙客来和商业 F1 Melody 品种的花药和胚珠培养开发有效的单倍体植物再生方案。使用C. persicum Mill的 DAPI（4',6-二脒基-2-苯基吲哚二盐酸盐）染料测定小孢子的单核阶段。和商业品种。离体前对芽进行冷预处理（4℃）2 d胚珠和花药培养物。花药在与不同剂量的1-萘乙酸(NAA)、135.0g L ^-1麦芽糖、硝酸银(AgNO ₃ )和活性炭(AC)组合的B5培养基上培养。胚珠在包含不同量的2,4-二氯苯氧基乙酸(2,4-D)和N6-(2-异戊烯基腺嘌呤)(2iP)和蔗糖的Murashige和Skoog (MS)培养基上培养。^{胚胎在M2培养基（含有0.2 mg L -1}赤霉酸（GA ₃）、0.1 mg L ^-1 6-苄氨基嘌呤（BA）、1.0 g L ^-1脯氨酸和0.05 mg L ^-1的MS）上成熟并发芽。精胺）用于花药培养，不含植物生长调节剂（PGR）的 MS 培养基用于胚珠培养。单倍体胚胎从B5培养基中获得，其中包含1.0mg L ^-1 NAA用于C. persicum Mill。（100%）。确定了C. persicum Mill的有效胚珠培养方案。为 2.0 mg L ^-1 2,4-D 和 0.8 mg L ^-1 2iP；Melody F1 品种的2.0 mg L ^-1 2,4-D 和 0.5 mg L ^{-1 2iP 为 100%。}在C. persicum Mill上检测到自发双单倍化。通过流式细胞术分析。将植物转移到土壤中，并在适应后4个月观察开花。