A dimeric indole alkaloid, isovincathicine consisting of an aspidosperma type and modified iboga with C-7–C-20 connection type skeletons was first isolated from Catharanthus roseus, and the structure including stereochemistry was elucidated on the basis of spectroscopic data as well as DP4 statistical analysis. Isovincathicine inhibited cell proliferation in A549 cells. We investigated the detailed mode of action of isovincathicine-induced inhibitory effects on cell proliferation in A549 cells. Flow cytometric analysis showed that isovincathicine-treated cells accumulated in the G₂ phase after 24 h, and the percentage of cells showing cell death increased after 48 h. Western blotting also showed increased expression of BimEL, an apoptosis-related protein, and decreased expression of Mcl-1 and Bcl-xL. Isovincathicine was suggested to induce apoptosis in A549 cells by a mechanism is similar to that of vinblastine.

Graphical abstract

中文翻译：

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长春花异长春碱诱导 A549 细胞凋亡

首次从长春花中分离出一种二聚吲哚生物碱异长春碱，其由蜘蛛型和具有 C-7-C-20 连接型骨架的修饰 iboga 组成，并根据光谱数据和 DP4 统计阐明了包括立体化学在内的结构。分析。异长春卡辛抑制 A549 细胞的细胞增殖。我们研究了异长春卡辛诱导的 A549 细胞增殖抑制作用的详细作用模式。流式细胞术分析表明，异长春卡辛处理的细胞在24小时后积累在G ₂期，并且在48小时后显示细胞死亡的细胞百分比增加。Western blotting 还显示 BimEL（一种凋亡相关蛋白）的表达增加，而 Mcl-1 和 Bcl-xL 的表达减少。异长春花碱被认为通过与长春花碱相似的机制诱导 A549 细胞凋亡。

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