Toxoplasmosis causes serious harm to the fetus, as tachyzoite dissemination, during pregnancy in women developing the primo-infection. The microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA. Circulating miRNA are promising biomarkers for diagnosis and prognosis of numerous diseases. The miRNAs levels are estimated by quantitative real-time PCR (qPCR), however, the relative quantification of each miRNA expression requires proper normalization methods using endogenous miRNAs as control. This study analyzed the expression of three endogenous miRNAs (miR-484, miR ‐423‐3p and miR-26b-5p) for use as normalizers in future studies of target miRNAs for gestational toxoplasmosis (GT). A total of 32 plasma samples were used in all assays divided in 21 from women with GT and 11 from healthy women. The stability of each endogenous miRNA was evaluated by the algorithm methods RefFinder that included GeNorm, Normfinder, BestKeeper and comparative delta-CT programs. The miR-484 was the most stably gene, and equivalently expressed in GT and NC groups. These results contribute to future studies of target miRNAs in clinical samples of women with gestational toxoplasmosis.

弓形虫病对胎儿造成严重危害，因为在怀孕期间发生初次感染的妇女会传播速殖子。microRNA (miRNA) 是小型非编码 RNA，通过沉默信使 RNA 在细胞中发挥调节作用。循环 miRNA 是用于许多疾病的诊断和预后的有前途的生物标志物。miRNA 水平通过定量实时 PCR (qPCR) 进行估计，然而，每个 miRNA 表达的相对定量需要使用内源 miRNA 作为对照的适当标准化方法。本研究分析了三种内源性 miRNA（miR-484、miR ‐423-3p 和 miR-26b-5p）的表达，作为未来妊娠弓形虫病 (GT) 靶标 miRNA 研究中的标准化因子。所有检测中总共使用了 32 份血浆样本，其中 21 份来自 GT 女性，11 份来自健康女性。每个内源 miRNA 的稳定性通过 RefFinder 算法方法进行评估，其中包括 GeNorm、Normfinder、BestKeeper 和比较 delta-CT 程序。miR-484是最稳定的基因，在GT组和NC组中表达相当。这些结果有助于未来对妊娠弓形虫病女性临床样本中目标 miRNA 的研究。