Colletotrichum gloeosporioides cause citrus anthracnose, is a major threat to citrus quality and fruit production worldwide. In the present study, different experiments were designed to check the potential of various chemicals and biocontrol agents (BCA) against C. gloeosporioides and characterize pathogen from core citrus growing regions of citrus in Punjab, Pakistan. Identification of different pathogens and phylogenetic relationship of Colletotrichum spp. was also performed to examine the relationship between isolates. The phylogenetic relationship determined across 16 C. gloeosporioides isolates showed five distinct clades, highlighting the importance of genetic variation among isolates. Pathogens were identified on the base of the internal transcribed spacers (ITS) region. During survey the disease incidence on fruits and leaves were identified and C. gloeosporioides found to be the most prevalent pathogens. Integrated management was done using BCA (Trichoderma harzianum and T. viride) and the synthetic combined fungicide (Cymoxanil+Mancozeb) under field conditions. Results revealed that, T. harzianum with T. viride and Cymoxanil+Mancozeb gave maximum inhibition against C. gloeosporioides and enhance plant performance under field conditions. In conclusion, the BCA (T. harzianum and T. viride) and the Cymoxanil+Mancozeb could be a better strategy to mitigate the heavy loss of citrus fruit due to anthracnose disease.

胶孢炭疽菌引起柑橘炭疽病，是全球柑橘质量和水果生产的主要威胁。在本研究中，设计了不同的实验来检查各种化学品和生物防治剂（BCA）对抗念珠菌的潜力。 gloeosporioides并描述了来自巴基斯坦旁遮普邦柑橘核心柑橘种植区的病原体。炭疽菌不同病原菌的鉴定及系统发育关系。还进行了检查分离株之间的关系。确定 16 个 C. gloeosporioides 的系统发育关系分离株显示出五个不同的进化枝，凸显了分离株之间遗传变异的重要性。在内转录间隔区 (ITS) 区域的基础上鉴定出病原体。调查期间确定了果实和叶子的发病率，C． 发现胶孢子虫是最流行的病原体。在田间条件下使用BCA（哈茨木霉和绿色木霉）和合成组合杀菌剂（霜脲氰+代森锰锌）进行综合管理。结果表明，哈茨木霉与绿色木霉以及霜脲氰+代森锰锌对念珠菌具有最大的抑制作用。 炭疽病并提高植物在田间条件下的性能。总之，BCA（哈茨木霉和绿色木霉）和霜脲氰+代森锰锌可能是减轻柑橘类水果炭疽病造成的严重损失的更好策略。