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CRISPR-Cas System, a Possible “Savior” of Rice Threatened by Climate Change: An Updated Review
Rice ( IF 5.5 ) Pub Date : 2023-09-09 , DOI: 10.1186/s12284-023-00652-1
Nabeel Shaheen 1 , Shakeel Ahmad 1 , Salem S Alghamdi 2 , Hafiz Mamoon Rehman 3 , Muhammad Arshad Javed 4 , Javaria Tabassum 4 , Gaoneng Shao 5, 6
Affiliation  

Climate change has significantly affected agriculture production, particularly the rice crop that is consumed by almost half of the world’s population and contributes significantly to global food security. Rice is vulnerable to several abiotic and biotic stresses such as drought, heat, salinity, heavy metals, rice blast, and bacterial blight that cause huge yield losses in rice, thus threatening food security worldwide. In this regard, several plant breeding and biotechnological techniques have been used to raise such rice varieties that could tackle climate changes. Nowadays, gene editing (GE) technology has revolutionized crop improvement. Among GE technology, CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein) system has emerged as one of the most convenient, robust, cost-effective, and less labor-intensive system due to which it has got more popularity among plant researchers, especially rice breeders and geneticists. Since 2013 (the year of first application of CRISPR/Cas-based GE system in rice), several trait-specific climate-resilient rice lines have been developed using CRISPR/Cas-based GE tools. Earlier, several reports have been published confirming the successful application of GE tools for rice improvement. However, this review particularly aims to provide an updated and well-synthesized brief discussion based on the recent studies (from 2020 to present) on the applications of GE tools, particularly CRISPR-based systems for developing CRISPR rice to tackle the current alarming situation of climate change, worldwide. Moreover, potential limitations and technical bottlenecks in the development of CRISPR rice, and prospects are also discussed.



中文翻译:

CRISPR-Cas系统,可能是受气候变化威胁的水稻的“救世主”:最新综述

气候变化严重影响了农业生产,特别是稻米作物,全球近一半人口消费着稻米作物,对全球粮食安全做出了重大贡献。水稻容易受到干旱、高温、盐度、重金属、稻瘟病和白叶枯病等多种非生物和生物胁迫的影响,导致水稻产量巨大损失,从而威胁全球粮食安全。在这方面,一些植物育种和生物技术已被用来培育能够应对气候变化的水稻品种。如今,基因编辑(GE)技术彻底改变了作物改良。在GE技术中,CRISPR/Cas(Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR相关蛋白)系统已成为最方便、稳健、成本效益高且劳动力密集程度较低的系统之一,因此越来越受欢迎植物研究人员,特别是水稻育种家和遗传学家。自 2013 年(基于 CRISPR/Cas 的 GE 系统首次在水稻中应用的一年)以来,利用基于 CRISPR/Cas 的 GE 工具开发了几种具有特定性状的气候适应型水稻品系。早些时候,已经发表的几份报告证实了转基因工具在水稻改良中的成功应用。然而,这篇综述的特别目的是根据最近(从 2020 年至今)有关 GE 工具应用的研究,特别是基于 CRISPR 的系统来开发 CRISPR 水稻,以解决当前令人担忧的情况,提供更新且全面的简要讨论。气候变化,全世界。此外,还讨论了CRISPR水稻发展的潜在局限性和技术瓶颈以及前景。

更新日期:2023-09-09
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