The proposal of the concept of Precision Science and the rapid development of technology have raised expectations for a higher demand for accurate quantitative analysis, including hair analysis in drug tests. Many details are presently constraining improvements in precision, so there is a greater need for standardized methods to control and improve the analytical process in the laboratory. Hair matrix reference materials may become a feasible option. In the present study, 34 drug-free hair samples from healthy volunteers were collected and compared with 2 certified standard hair samples. It was observed that the hair matrix of different origins did have a significant impact on hair analysis. Three reference materials were prepared using spiking and two soaking methods, and one natural reference material was collected from real cases. A total of 57 authentic hair samples were measured from March to August 2022 using these 4 reference materials. Differences in the origins of samples were found to have a significant impact on the hair analysis results. The reference material soaked in water and methanol at a 1:1 ratio (v/v) appeared to be an excellent tool for daily quality control, as it gave results closest to those of the natural matrix reference material in all aspects and supported the law principle of the Presumption of Innocence.

精密科学概念的提出和技术的快速发展，对精准定量分析提出了更高的要求，包括药物测试中的毛发分析。目前，许多细节限制了精度的提高，因此更需要标准化方法来控制和改进实验室的分析过程。头发基质参考材料可能成为一种可行的选择。在本研究中，收集了来自健康志愿者的 34 份不含药物的头发样本，并与 2 份经过认证的标准头发样本进行了比较。据观察，不同来源的毛发基质确实对毛发分析有显着影响。采用加标和两种浸泡方法制备了三种标准物质，并从真实案例中收集了一种天然标准物质。使用这 4 种参考材料，从 2022 年 3 月至 8 月总共测量了 57 个真实的头发样本。研究发现样品来源的差异对头发分析结果有显着影响。以 1:1 (v/v) 比例浸泡在水和甲醇中的标准物质似乎是日常质量控制的绝佳工具，因为它给出的结果在各方面最接近天然基质标准物质，并支持该定律无罪推定原则。