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Implementing ATR-FTIR spectroscopy and chemometrics for the forensic examination of sexual lubricants and their traces
Forensic Chemistry ( IF 2.7 ) Pub Date : 2023-09-09 , DOI: 10.1016/j.forc.2023.100533
Chongtham Nimi , Arti Yadav , Parampreet Singh , Sweety Sharma , Rajinder Singh

Sexual lubricants are likely to be encountered at crime scenes due to the wide availability and variability of the products. These lubricants found in sexual assault cases may help supplement biological evidence or be the primary evidence where there is a lack of DNA evidence. The discrimination of sexual lubricants will help assess the potential contact between the victim and the suspect. In this study, an analysis of a total of 43 products of condom lubricants, bottled sexual lubricants, and personal hygiene products was carried out to evaluate their variability and discrimination potential. ATR-FTIR spectroscopy was used to analyze the samples, and the spectra were then visually examined and interpreted using chemometrics. Visual analysis revealed three different groupings of the samples based on their chemical profile. Chemometric classification was done using LDA and SVM in a two-stage classification process: identification of the type of product and a brand-level classification. The combination of LDA and SVM helped to discriminate the samples further in an objective manner. In stage 1 classification, SVM showed 100% classification accuracy. In the second stage, 70.83% classification accuracy was achieved using SVM for condom samples. Whereas, 96.15% classification was achieved using LDA for bottled lubricants. Evaluation of how these products can be linked to their sources is also needed when they are encountered as traces in various substrates. A substrate study was conducted while considering two factors: environment and time. The findings indicated that the type of substrate, lubricant, and storage period may affect the discrimination.



中文翻译:

采用 ATR-FTIR 光谱和化学计量学对性润滑剂及其痕迹进行法医检查

由于产品的广泛可用性和多样性,在犯罪现场很可能会遇到性润滑剂。在性侵犯案件中发现的这些润滑剂可能有助于补充生物证据,或者在缺乏 DNA 证据的情况下成为主要证据。性润滑剂的区分将有助于评估受害者和嫌疑人之间的潜在接触。在这项研究中,对避孕套润滑剂、瓶装性润滑剂和个人卫生用品等总共 43 种产品进行了分析,以评估它们的变异性和区分潜力。使用 ATR-FTIR 光谱分析样品,然后使用化学计量学对光谱进行目视检查和解释。视觉分析根据化学特征揭示了样品的三种不同分组。化学计量学分类是使用 LDA 和 SVM 在两阶段分类过程中完成的:产品类型识别和品牌级分类。LDA和SVM的结合有助于进一步客观地判别样本。在第 1 阶段分类中,SVM 显示出 100% 的分类准确率。第二阶段,使用SVM对避孕套样本进行分类,准确率达到70.83%。而使用 LDA 对瓶装润滑油实现了 96.15% 的分类。当这些产物在各种基质中以痕迹形式出现时,还需要评估如何将它们与其来源联系起来。进行底物研究时考虑了两个因素:环境和时间。研究结果表明,基材的类型、润滑剂和储存期可能会影响辨别力。

更新日期:2023-09-09
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