Glucosinolates (GSLs) are secondary metabolites that are produced in Brassicale order plants and play an important role in their defense against biotic and abiotic stresses. In addition, GSLs were reported to possess health benefits like anticancer and anti-inflammation activity. In the present study, the GSL profiles of 13 radishes (Raphanus sativus L) cultivars or inbred lines with different root phenotypes were analyzed. A total of four aliphatics and three indolic GSL compounds were consistently detected from all tested radish accessions. Based on the amounts and profiles of the GSL compounds, we identified that glucoraphasatin (GRH) and glucobrassicin (GBS) were the most abundant in the aliphatic and indolic GSLs, respectively, in young radishes. In addition, we performed a correlation heatmap analysis between the profiles of the individual GSL compounds and the GSL biosynthetic genes involved in the secondary modifications. We found that RsGRS1 plays a major role in the highest abundance of GRH in the aliphatic GSLs. Contrastingly, RsCYP81F3 and its paralogs, RsCYP81Fs might contribute to the profiles of the indolic GSLs in young radishes. In this study, we found that there was a substantial correlation between the transcription of the genes involved in the secondary modification phase of the GSL biosynthetic pathway and the profiles of the aliphatic and indolic GSL compounds.

芥子油苷 (GSL) 是芸苔目植物中产生的次生代谢产物，在抵御生物和非生物胁迫方面发挥着重要作用。此外，据报道，GSL 具有抗癌和抗炎活性等健康益处。在本研究中，13 个萝卜（Raphanus sativusL) 分析了具有不同根表型的品种或自交系。从所有测试的萝卜种质中一致检测到总共四种脂肪族化合物和三种吲哚族 GSL 化合物。根据 GSL 化合物的含量和分布，我们发现幼萝卜中的脂肪族 GSL 和吲哚 GSL 中分别最丰富的是葡萄糖苷 (GRH) 和芸苔素 (GBS)。此外，我们还对各个 GSL 化合物的概况和参与二次修饰的 GSL 生物合成基因之间进行了相关热图分析。我们发现 RsGRS1 在脂肪族 GSL 中 GRH 丰度最高的过程中发挥着重要作用。相比之下，RsCYP81F3及其旁系同源物RsCYP81Fs可能有助于幼萝卜中吲哚 GSL 的分布。在这项研究中，我们发现参与 GSL 生物合成途径二次修饰阶段的基因转录与脂肪族和吲哚 GSL 化合物的谱之间存在显着相关性。