Abstract

Mobile genetic elements (MGEs) are the DNA sequences capable of moving inside the host genome. As a result of ongoing mutation processes and low natural selection pressure, the evolutionary diversity of MGEs is constantly increasing. Eukaryotic DNA transposons represent a very large and diverse group of MGEs. This work was focused on the study of the pogo DNA transposons in three genomes of jellyfish in the genus Aurelia: Aurelia aurita, Aurelia coerulea, and Aurelia aurita complex sp. In this work, the local alignment method (BLASTn) was used to search for the pogo group MGEs. Multiple amino-acid sequence alignment was performed using MAFFT. It was demonstrated that the majority of pogo elements were represented by the Fot/Fot-like family elements. Two new subfamilies, auFLE1 and auFLE2, were identified in this group. The Jelly group including two elements was also detected in jellyfish. No potentially functional DNA transposons were found among the detected pogo elements. Analysis of the identified pogo elements showed that no active DNA transposons were preserved in the studied jellyfish genomes: all elements are damaged by mutations or have deletions and are not represented by full-size copies. This allowed us to suggest that the activity peak for these transposons took place relatively long ago.

中文翻译：

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Aurelia 属水母基因组中的 Pogo DNA 转座子

摘要

移动遗传元件 (MGE) 是能够在宿主基因组内移动的 DNA 序列。由于持续的突变过程和较低的自然选择压力，MGE 的进化多样性不断增加。真核 DNA 转座子代表了一个非常大且多样化的 MGE 群体。这项工作的重点是研究Aurelia属水母的三个基因组中的pogo DNA 转座子：Aurelia aurita、Aurelia coerulea和Aurelia aurita complex sp。在这项工作中，使用局部对齐方法（BLASTn）来搜索pogoMGE 组。使用MAFFT进行多氨基酸序列比对。结果表明，大多数pogo元素都是由Fot/Fot 类元素代表的。在该组中鉴定出两个新的亚家族，auFLE1和auFLE2 。在水母中也检测到了包含两种元素的果冻族。在检测到的pogo元件中没有发现具有潜在功能的 DNA 转座子。对已识别的pogo的分析元件显示，所研究的水母基因组中没有保留活性DNA转座子：所有元件都因突变而损坏或有缺失，并且不以全尺寸拷贝表示。这使我们能够表明这些转座子的活动峰值发生在相对很久以前。