Abstract

Hantaviruses (genus Orthohantavirus, family Hantaviridae) have been identified worldwide and cause two kinds of human disease. In recent years, genetic evidence of pathogenicity for humans has been demonstrated for Tula virus, circulating in Europe among common voles (Microtus arvalis). The goal of the study consisted in detection of natural foci of hantavirus infection among voles in Siberia and genetic identification of vole-borne hantaviruses. A total of 254 rodents of the Arvicolinae subfamily were captured in West and East Siberia and tested for hantavirus-specific antibodies by immunofluorescent assay and for hantavirus RNA using reverse transcription polymerase chain reaction. results. Hantavirus antibody-positive rodents were found in Altai and Krasnoyarsk krai, Tomsk, Novosibirsk oblast, and the Republic of Altai among root voles (Alexandromys oeconomus), field voles (Microtus agrestis), narrow-headed voles (Lasiopodomys gregalis), and steppe lemmings (Lagurus lagurus). Viral RNA was detected and identified as Tula virus in two species of seropositive animals. Phylogenetic analysis showed that Siberian strains of Tula virus represent distinct genetic variants, Russia II and Russia III, associated with narrow-headed voles and steppe lemmings, respectively. Our data demonstrated that Tula virus is present from Altai krai, Tomsk, Novosibirsk oblast in West Siberia up to East Siberia (Krasnoyarsk krai) in Asian Russia.

中文翻译：

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西伯利亚田鼠种群中的汉坦病毒

摘要

汉坦病毒（汉坦病毒科，正汉坦病毒属）已在全世界范围内被发现，可引起两种人类疾病。近年来，图拉病毒对人类具有致病性的遗传证据已得到证实，该病毒在欧洲普通田鼠（Microtus arvalis）中传播）。该研究的目的包括检测西伯利亚田鼠中汉坦病毒感染的自然病灶以及田鼠传播的汉坦病毒的基因鉴定。在西西伯利亚和东西伯利亚捕获了总共 254 只 Arvicolinae 亚科啮齿动物，并通过免疫荧光测定法检测汉坦病毒特异性抗体，并使用逆转录聚合酶链反应检测汉坦病毒 RNA。结果。在阿尔泰和克拉斯诺亚尔斯克边疆区、托木斯克、新西伯利亚州和阿尔泰共和国的根田鼠（Alexandromys oeconomus）、田鼠（Microtus agrestis）、窄头田鼠（Lasiopodomys gregalis）和草原旅鼠中发现了汉坦病毒抗体阳性的啮齿动物（黑尾狐猴）。在两种血清反应呈阳性的动物中检测到病毒 RNA，并鉴定为图拉病毒。系统发育分析表明，图拉病毒的西伯利亚毒株代表了不同的遗传变异，即俄罗斯II型和俄罗斯III型，分别与窄头田鼠和草原旅鼠相关。我们的数据表明，图拉病毒存在于西西伯利亚的阿尔泰边疆区、托木斯克州、新西伯利亚州至俄罗斯亚洲地区的东西伯利亚（克拉斯诺亚尔斯克边疆区）。