Spinibarbus hollandi (Cyprinidae, Barbinae) is an economically important species and widely distributed in numbers of rivers throughout South China. However, wild S. hollandi resources have declined sharply in recent years as a result of over fishing, environmental pollution and other human disturbance. Therefore, it is extremely urgent to protect germplasm resource of S. hollandi. In this study, 32 novel single nucleotide polymorphism (SNP) markers were first developed based on restriction site-associated DNA sequencing (RAD-seq). The results showed that the minor allele frequency (MAF) varied from 0.010 to 0.337, the observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.000 to 0.127 and 0.043 to 0.402, respectively. The PIC value varied from 0.048 to 0.375. Only two loci revealed significant departures from Hardy-Weinberg equilibrium. These novel SNP markers will contribute a lot for future studies on population structure and conservation genetics of this species.

Spinibarbus hollandi（鲤科，Barbinae）是一种重要的经济物种，广泛分布于华南地区的多条河流。然而，近年来，由于过度捕捞、环境污染和其他人为干扰，野生霍兰迪资源急剧减少。因此，保护​​霍兰迪种质资源刻不容缓。在这项研究中，首先基于限制性位点相关 DNA 测序 (RAD-seq) 开发了 32 个新型单核苷酸多态性 (SNP) 标记。结果显示，次要等位基因频率（MAF）范围为0.010至0.337，观察杂合度（Ho）和预期杂合度（He）分别为0.000至0.127和0.043至0.402。PIC 值从 0.048 到 0.375 不等。只有两个基因座显着偏离哈迪-温伯格平衡。这些新的SNP标记将对该物种的种群结构和保护遗传学的未来研究做出很大贡献。