The pharmacokinetics were described of meloxicam (MLX) in green sea turtles (Chelonia mydas), following a single intravenous (i.v.) and intramuscular (i.m.) administrations at one of two dosages of 0.1 or 0.2 mg/kg body weight (b.w.). The sample of 20 green sea turtles was divided into four groups (n = 5) using a randomization procedure according to a parallel study design. Blood samples were collected at pre-assigned times up to 168 h. MLX in the plasma was cleaned-up and quantified using a validated high-performance liquid chromatography method with UV detection. The concentration of MLX in the experimental green sea turtles with respect to time was pharmacokinetically analyzed using a non-compartment model. MLX plasma concentrations were quantifiable for up to 72 and 120 h after i.v. at dosages of 0.1 and 0.2 mg/kg b.w., respectively, whereas it was measurable for up to 168 h after i.m. administration at both dosages. The long elimination half-life value of MLX (28 h) obtained in green sea turtles after i.v. administration was consistent with the quite slow clearance rate for both dosages. The average maximum concentration (C_max) values of MLX were 1.05 μg/mL and 4.26 μg/mL at dosages of 0.1 and 0.2 mg/kg b.w., respectively, with their elimination half-life values being 37.26 h and 30.64 h, respectively, after i.m. administrations. The absolute i.m. bioavailability was approximately 110%. These results suggested that i.m. administration of MLX at a dosage of 0.2 mg/kg b.w. was likely to be effective for clinical use in green sea turtles (Chelonia mydas). However, further studies are needed to determine the pharmacodynamic properties and clinical efficacy of MLX for the treatment of inflammatory disease after single and multiple dosages.

中文翻译：

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静脉内和肌肉内给药后美洛昔康在绿海龟 (Chelonia mydas) 中的处置动力学

描述了美洛昔康 (MLX) 在绿海龟 ( Chelonia mydas ) 中的药代动力学，以 0.1 或 0.2 mg/kg 体重 (bw) 的两种剂量之一进行单次静脉内 (iv) 和肌内 (im) 给药。 根据平行研究设计，使用随机化程序将 20 只绿海龟样本分为四组 ( n = 5)。在预先指定的时间采集血样，最长可达 168 小时。使用经过验证的高效液相色谱法和 UV 检测对血浆中的 MLX 进行净化和定量。使用非房室模型对实验绿海龟中 MLX 浓度随时间的变化进行药代动力学分析。静脉注射0.1和0.2毫克/千克体重剂量后，MLX血浆浓度分别可在72和120小时内进行定量，而两种剂量的肌肉注射后，可在168小时内进行测量。绿海龟静脉注射后获得的 MLX 长消除半衰期值（28 小时）与两种剂量的相当缓慢的清除率一致。MLX在0.1和0.2 mg/kg体重剂量下的平均最大浓度（C _max）值分别为1.05 μg/mL和4.26 μg/mL，其消除半衰期值分别为37.26 h和30.64 h。在我管理之后。绝对im生物利用度约为110%。这些结果表明，以0.2mg/kg体重的剂量肌内施用MLX对于绿海龟( Chelonia mydas )的临床应用可能是有效的。然而，还需要进一步的研究来确定 MLX 单次和多次剂量治疗炎症性疾病的药效学特性和临床疗效。