One of the primary corn diseases, banded leaf and sheath blight, is carried on by the pathogenic fungus Rhizoctonia solani. Efforts to control R. solani are more directed at environmentally friendly control using a biological control agent, such as Trichoderma spp. as antagonistic agents and plant growth promoter. This study aimed to identify T. asperellum isolates based on molecular characteristics and to determine the in vitro inhibition mechanism against R. solani and its role in enhancing the growth of corn seedlings. The HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01 isolates were identified as T. asperellum with a genetic distance coefficient value of 0.000 and a very high similarity of 100%. Meanwhile, the RsHM isolate was identified as R. solani with a homology level of > 90% and genetic distance coefficient values ranging from 0.000 to 0.032. The antagonistic ability showed that the five T. asperellum isolates were able to inhibit the growth of R. solani in vitro on PDA medium with an inhibition percentage of ≥ 50%, so they were categorized as antagonist agents. T. asperellum showed the antagonistic mechanism in inhibiting the R. solani growth through the action of parasitism. The five T. asperellum isolates tested on corn seedlings showed a significantly high difference from the control treatment on the observational variables of maximum growth potential (MGP), growth rate (GtR), growth simultaneity (GS), vigor index (VI), germination rate (GR), and median germination time (T50). CHM01 isolate showed better potential than other isolates in inhibiting the growth of R. solani in vitro on PDA medium with a parasitism mechanism and enhancing the growth of corn seedlings.

中文翻译：

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玉米木霉对条斑叶斑病和纹枯病的立枯丝核菌的体外抑制机制及其促进玉米幼苗生长的作用

玉米的主要病害之一是带叶纹枯病，由病原真菌立枯丝核菌 (Rhizoctonia solani) 传播。控制立枯丝核菌的努力更多地针对使用生物防治剂（例如木霉属）进行环境友好的控制。作为拮抗剂和植物生长促进剂。本研究旨在根据分子特征鉴定曲霉属菌株，并确定其对立枯丝霉的体外抑制机制及其促进玉米幼苗生长的作用。HMRP7、HMRF7A、HMEDF1B、HMEDF6A 和 CHM01 分离株被鉴定为 T. asperellum，遗传距离系数值为 0.000，相似性极高，达到 100%。同时，RsHM分离物被鉴定为R. solani，其同源性水平> 90%，遗传距离系数值范围为0.000至0。032. 拮抗能力表明，5株曲霉菌株在体外PDA培养基上均能抑制立枯病菌的生长，抑制率≥50%，属于拮抗剂。T. asperellum表现出通过寄生作用抑制茄病菌生长的拮抗机制。在玉米幼苗上测试的 5 个 T. asperellum 分离株在最大生长潜力 (MGP)、生长速率 (GtR)、生长同时性 (GS)、活力指数 (VI)、发芽等观察变量上与对照处理显示出显着的差异。率（GR）和中位发芽时间（T50）。CHM01分离株在具有寄生机制的PDA培养基上体外抑制立枯丝核菌生长并促进玉米幼苗生长方面比其他分离株表现出更好的潜力。拮抗能力表明，5株曲霉菌株在体外PDA培养基上均能抑制立枯病菌的生长，抑制率≥50%，属于拮抗剂。T. asperellum表现出通过寄生作用抑制茄病菌生长的拮抗机制。在玉米幼苗上测试的 5 个 T. asperellum 分离株在最大生长潜力 (MGP)、生长速率 (GtR)、生长同时性 (GS)、活力指数 (VI)、发芽等观察变量上与对照处理显示出显着的差异。率（GR）和中位发芽时间（T50）。CHM01分离株在具有寄生机制的PDA培养基上体外抑制立枯丝核菌生长并促进玉米幼苗生长方面比其他分离株表现出更好的潜力。拮抗能力表明，5株曲霉菌株在体外PDA培养基上均能抑制立枯病菌的生长，抑制率≥50%，属于拮抗剂。T. asperellum表现出通过寄生作用抑制茄病菌生长的拮抗机制。在玉米幼苗上测试的 5 个 T. asperellum 分离株在最大生长潜力 (MGP)、生长速率 (GtR)、生长同时性 (GS)、活力指数 (VI)、发芽等观察变量上与对照处理显示出显着的差异。率（GR）和中位发芽时间（T50）。CHM01分离株在具有寄生机制的PDA培养基上体外抑制立枯丝核菌生长并促进玉米幼苗生长方面比其他分离株表现出更好的潜力。