Cells orchestrate histone biogenesis with strict temporal and quantitative control. To efficiently regulate histone biogenesis, the repetitive Drosophila melanogaster replication-dependent histone genes are arrayed and clustered at a single locus. Regulatory factors concentrate in a nuclear body known as the histone locus body (HLB), which forms around the locus. Historically, HLB factors are largely discovered by chance, and few are known to interact directly with DNA. It is therefore unclear how the histone genes are specifically targeted for unique and coordinated regulation. To expand the list of known HLB factors, we performed a candidate-based screen by mapping 30 publicly available ChIP datasets of 27 unique factors to the Drosophila histone gene array. We identified novel transcription factor candidates, including the Drosophila Hox proteins Ultrabithorax (Ubx), Abdominal-A (Abd-A), and Abdominal-B (Abd-B), suggesting a new pathway for these factors in influencing body plan morphogenesis. Additionally, we identified six other factors that target the histone gene array: JIL-1, hormone-like receptor 78 (Hr78), the long isoform of female sterile homeotic (1) (fs(1)h) as well as the general transcription factors TBP associated factor 1 (TAF-1), Transcription Factor IIB (TFIIB), and Transcription Factor IIF (TFIIF). Our foundational screen provides several candidates for future studies into factors that may influence histone biogenesis. Further, our study emphasizes the powerful reservoir of publicly available datasets, which can be mined as a primary screening technique.

中文翻译：

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生物信息学筛选揭示果蝇组蛋白基因座的 hox 和染色质重塑因子

细胞通过严格的时间和定量控制来协调组蛋白的生物发生。为了有效调节组蛋白生物合成，重复的黑腹果蝇复制依赖性组蛋白基因被排列并聚集在单个基因座上。调节因子集中在称为组蛋白基因座体 (HLB) 的核体中，该核体在基因座周围形成。从历史上看，HLB 因子很大程度上是偶然发现的，很少有人知道它与 DNA 直接相互作用。因此，尚不清楚组蛋白基因如何专门针对独特且协调的调节。为了扩展已知 HLB 因子的列表，我们通过将 27 个独特因子的 30 个公开可用的 ChIP 数据集映射到果蝇组蛋白基因阵列来进行基于候选的筛选。我们鉴定了新的候选转录因子，包括果蝇 Hox 蛋白 Ultrabithorax (Ubx)、Abdominal-A (Abd-A) 和 Abdominal-B (Abd-B)，表明这些因子影响身体计划形态发生的新途径。此外，我们还确定了其他六个针对组蛋白基因阵列的因子：JIL-1、激素样受体 78 (Hr78)、雌性不育同源异构体 (1) (fs(1)h) 的长亚型以及一般转录TBP 相关因子 1 (TAF-1)、转录因子 IIB (TFIIB) 和转录因子 IIF (TFIIF)。我们的基础筛选为未来研究可能影响组蛋白生物发生的因素提供了几种候选者。此外，我们的研究强调了公开数据集的强大库，可以将其作为主要筛选技术进行挖掘。