Wastewater generated by textile industry needs to be treated to reduce its toxicity before final disposal and/or for recycling purposes. In the current study, several bacterial strains were screened for dye decolorization potential. UV–visible spectroscopy was used to determine maximum absorption wavelength of disperse dyes. HPLC and MTS assay were used to confirm the degradation and detoxification of disperse dyes, respectively. Results revealed that indigenously isolated Bacillus licheniformis, Glutamicibacter uratoxydans and Pseudomonas aeruginosa showed strong decolorization of red, blue and violet, respectively in 6–9 h. MTS assay revealed 100% viability of NIH/3T3 cell lines in presence of treated dyes. Enzyme screening assay confirmed the production of intracellular and membrane bound oxidoreductases in presence of specific dye as substrate. To resolve this issue, bacterial consortia were prepared, and better decolorization of all dyes was achieved in synergistic behaviour of Consortia 1 and 4 with 85% and 88% decolorization potential, respectively.

中文翻译：

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本地分离的细菌单一培养物对偶氮和蒽醌染料的酶促生物降解和解毒及其在发达联盟中的协同行为

纺织工业产生的废水在最终处置和/或回收之前需要进行处理以降低其毒性。在当前的研究中，筛选了几种细菌菌株的染料脱色潜力。紫外可见光谱用于测定分散染料的最大吸收波长。 HPLC和MTS测定分别用于确认分散染料的降解和解毒作用。结果显示，本土分离的地衣芽孢杆菌、尿酸谷氨酸杆菌和铜绿假单胞菌分别在 6-9 小时内表现出强烈的红色、蓝色和紫色脱色作用。 MTS 测定显示 NIH/3T3 细胞系在处理过的染料存在下具有 100% 的活力。酶筛选测定证实了在特定染料作为底物存在下细胞内和膜结合氧化还原酶的产生。为了解决这个问题，制备了细菌菌群，并且菌群1和4的协同行为实现了所有染料的更好脱色，分别具有85%和88%的脱色潜力。