A cross-sectional study was carried out to determine the frequency and factors associated with infection in pet dogs in the metropolitan area of the Colombian Caribbean (northern Colombia). A total of 173 dogs were analyzed by a commercial rapid immunochromatographic test (RIT) and a nested PCR of the cytochrome oxidase subunit I gene, in parallel. Ninety-two (53.2%) of the dogs showed positive results to the RIT, while 59 (34.1%) animals had DNA by PCR. Positivity to one or both diagnostic techniques was detected in 104 (60.1%; CI: 53.8–67.4) of the sampled dogs. In PCR-positive dogs, phylogenetic analyses evidenced high nucleotide identity (100%) with sequences previously obtained from mosquitoes, dogs and other mammals in different countries. Exercise intolerance ( = 0.002; OR: 2.33; CI: 1.37–3.96) and thrombocytopenia ( = < 0.001; OR: 1.95; CI: 1.11–3.43) were the main factors associated with infection in dogs. The high frequency of in dogs indicates a wide distribution of this parasite in the metropolitan area of the Colombian Caribbean, which can be of animal and public health concern. Our results highlight the need to combine different methods to increase the diagnostic accuracy of .

中文翻译：

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哥伦比亚加勒比海大都市区宠物狗的恶丝虫

进行了一项横断面研究，以确定哥伦比亚加勒比海大都市区（哥伦比亚北部）宠物狗感染的频率和相关因素。通过商业快速免疫层析测试 (RIT) 和细胞色素氧化酶亚基 I 基因的巢式 PCR 并行分析了总共 173 只狗。92 只 (53.2%) 的狗的 RIT 结果呈阳性，而 59 只 (34.1%) 的狗通过 PCR 检测出 DNA。104 只（60.1%；CI：53.8–67.4）的样本狗检测到一种或两种诊断技术呈阳性。在 PCR 阳性的狗中，系统发育分析证明其与之前从不同国家的蚊子、狗和其他哺乳动物获得的序列具有高度的核苷酸同一性 (100%)。运动不耐受（= 0.002；OR：2.33；CI：1.37-3.96）和血小板减少症（= < 0.001；OR：1.95；CI：1.11-3.43）是与犬感染相关的主要因素。狗体内的高频率表明这种寄生虫在哥伦比亚加勒比海大都市区广泛分布，这可能会引起动物和公共卫生问题。我们的结果强调需要结合不同的方法来提高诊断的准确性。