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A dirty job: dishwasher wastewater reuse and upcycle through an ad hoc engineered microbial consortium
npj Clean Water ( IF 11.4 ) Pub Date : 2023-09-21 , DOI: 10.1038/s41545-023-00280-8
Annamaria Alabiso , Sara Frasca , Valerio Cantelmo , Marco Maria D’Andrea , Roberto Braglia , Francesco Scuderi , Fiammetta Costa , Saverio Savio , Roberta Congestri , Luciana Migliore

In the framework of regenerative circular economy and Sustainable Development Goals #6, water demand and wastewater production are a crucial environmental issue. To cope with this, a system called Zero Mile has been conceived to reuse/upcycle the dishwasher wastewater in both fertilisation of a vertical garden (for home plant production at zero mile) and the subsequent dishwasher rinsing cycle. The core of the Zero Mile system is a biofilter constituted by an ad hoc engineered microbial consortium, based on the mutualistic relationship between autotrophic and heterotrophic microbial partners, that makes the biodegradative process sustainable over time. Two semi-batch tests were performed to evaluate the capability and the efficiency of the microbial consortium to operate in conditions mimicking the Zero Mile system: consortia were challenged in two dishwasher wastewater volumes (50 and 500 mL), with a replenishing rate of 4 days for ¼ of the dishwasher wastewater volume. The results of these tests showed that the consortium thrives well in dishwasher wastewater over time and can survive with the volume and rates of replenishments. Next Generation Sequencing showed that the microbial consortium maintains a stable taxonomic composition, and chemical analyses demonstrated a very high bioremediation efficiency (total nitrogen and phosphorus removal). Aiming to contribute to the circular economy approach, the consortium ‘treated’ dishwasher wastewater was useful for plant fertilisation, as demonstrated by pigment content and quality indices (total phenols and flavonoids, soluble solids) measured in the lettuce plants grown in the Zero Mile demonstrator and watered with consortium reclaimed dishwasher wastewater.



中文翻译:

一项肮脏的工作:通过特设工程微生物联盟对洗碗机废水进行再利用和升级循环

在再生循环经济和可持续发展目标#6的框架下,水需求和废水产生是一个至关重要的环境问题。为了解决这个问题,一个名为“零英里”的系统被设计出来,用于在垂直花园的施肥(用于零英里的家庭植物生产)和随后的洗碗机冲洗循环中重复利用/升级利用洗碗机废水。零英里系统的核心是一个生物过滤器,由特设工程微生物群组成,基于自养和异养微生物伙伴之间的互惠关系,使生物降解过程随着时间的推移可持续。进行了两次半批量测试,以评估微生物群落在模仿零英里系统的条件下运行的能力和效率:联盟面临两种洗碗机废水量(50 和 500 mL)的挑战,1/4 洗碗机废水量的补充率为 4 天。这些测试的结果表明,随着时间的推移,该联盟在洗碗机废水中蓬勃发展,并且可以在补充量和补充率下生存。下一代测序显示微生物群落保持稳定的分类组成,化学分析表明具有非常高的生物修复效率总氮、总磷去除)。旨在为循环经济方法做出贡献,联盟“处理”的洗碗机废水可用于植物施肥,零英里示范点中生长的生菜植物中测量的色素含量和质量指数(总酚和类黄酮、可溶性固体)证明了这一点并用财团回收的洗碗机废水浇水。

更新日期:2023-09-22
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