Abstract

We analyzed the polymorphism of the complete mtDNA cytochrome b gene (1140 bp) and 12 microsatellite nDNA loci of the eastern red deer Cervus elaphus subspecies—Siberian C. e. sibiricus and Far East C. e. xanthopygus wapiti. On the territory of Russia, 112 samples were collected from different parts of the range, including Yakutia, where individuals with intermediate morphometric traits were found. We described 41 haplotypes, with no common haplotypes found for Siberian and Far East wapiti. Phylogenetic cytochrome b analysis revealed traces of ancestral polymorphism or introgression from Far East wapiti into Siberian wapiti. The red deer of Yakutia formed two haplogroups: the first group was genetically closer to Siberian wapiti, and the second was closer to American wapiti. The latter may indicate the preservation of ancient lines in Yakutia, which participated in the North America colonization during the Pleistocene glaciation. Microsatellite analysis revealed a little differentiation between the subspecies (Fst = 0.037), reflecting the presence of a constant gene flow between their populations. The genotypes of red deer from Yakutia demonstrate heterogeneity, indicating their mixed origin.

中文翻译：

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mtDNA和微卫星多态性揭示俄罗斯马鹿东部亚种遗传多样性

摘要

我们分析了东部马鹿马鹿亚种西伯利亚C. e的完整mtDNA细胞色素b基因（1140 bp）和12个微卫星nDNA位点的多态性。西伯利亚和远东C. e. xanthopygus马鹿。在俄罗斯境内，从包括雅库特在内的不同地区采集了 112 个样本，在那里发现了具有中间形态特征的个体。我们描述了 41 种单倍型，但没有发现西伯利亚和远东马鹿的常见单倍型。系统发育细胞色素b分析揭示了祖先多态性或从远东马鹿渗入西伯利亚马鹿的痕迹。雅库特的马鹿形成了两个单倍群：第一组在基因上更接近西伯利亚马鹿，第二组更接近美洲马鹿。后者可能表明雅库特保存了古代线，这些线在更新世冰川期间参与了北美殖民。微卫星分析显示亚种之间存在微小差异（Fst = 0.037），反映出其种群之间存在恒定的基因流。雅库特马鹿的基因型表现出异质性，表明它们的混合起源。