The infectious bursal disease virus (IBDV) causes an acute and highly contagious immunosuppressive response in young chickens by targeting B lymphocytes in immune organs. Changes in regulatory T-cell ratio and apoptosis have been demonstrated during IBDV infection in these cells. The possible change in CD19 expression as the precursor of B cells after IBDV replication was detected in this study. Raji cells were infected with an IBDV isolate at MOIs of 1.0 and 3.0. The viral kinetics were determined using the characteristic virus-induced CPE, cell viability, and infectious titer. Induction of apoptosis and also changes in the CD19 expression within the virus infection were assessed by flow cytometry. The Raji cells were found to be susceptible to IBDV infection by producing marked CPEs dependent on MOI. The infectivity titers were determined in intra- and extracellular samples at the defined hours. The kinetics of early IBDV replication in Raji cells were nearly identical for both MOIs, but a significant difference in the infectivity titer was observed at 48 hpi. The quick apoptotic events were observed to be significantly higher in MOI 3.0, which was correlated with the lower virus titer. A significant CD19 expression change in the IBDV-infected Raji cells was revealed. The results suggested that Raji cells mimic the IBDV replication in lymphoid organs and the virus replication is related to CD19 expression frequencies in the lymphoid cells.

传染性法氏囊病病毒 (IBDV) 通过靶向免疫器官中的 B 淋巴细胞，在幼鸡中引起急性、高度传染性的免疫抑制反应。IBDV 感染这些细胞期间，调节​​性 T 细胞比例和细胞凋亡发生了变化。本研究检测了IBDV复制后B细胞前体CD19表达可能发生的变化。Raji 细胞被 IBDV 分离株以 1.0 和 3.0 的 MOI 感染。使用特征病毒诱导的 CPE、细胞活力和感染滴度确定病毒动力学。通过流式细胞术评估病毒感染内细胞凋亡的诱导以及 CD19 表达的变化。发现 Raji 细胞通过产生依赖于 MOI 的标记 CPE 而容易受到 IBDV 感染。在规定的时间内测定细胞内和细胞外样品的感染滴度。对于两种 MOI，Raji 细胞中早期 IBDV 复制动力学几乎相同，但在 48 hpi 时观察到感染滴度存在显着差异。MOI 3.0 时观察到快速凋亡事件显着较高，这与较低的病毒滴度相关。感染 IBDV 的 Raji 细胞中 CD19 表达发生显着变化。结果表明，Raji细胞模仿淋巴器官中IBDV的复制，并且病毒复制与淋巴细胞中CD19的表达频率相关。