There is a wide variety of cancer cells that can be linked to the presence of TPX2. However, there is not a lot of evidence regarding its role in the development and maintenance of clear cell renal cell carcinoma (ccRCC). In our study, bioinformatics analysis was performed to obtain differentially expressed mRNAs and miR-NAs in ccRCC. Survival curves predicted correlation of TPX2 expression with patient survival. The upstream regulatory miRNA of TPX2 was predicted to be miRNA-27b-3p through database, and dual luciferase assay verified the targeted relationship. qRT-PCR and Western blot were employed for examination of TPX2 mRNA and protein expression in ccRCC cells. Proliferation, invasion, migration and cell cycle were detected by CCK-8, colony formation, wound healing, Transwell, and flow cytometry assays. The results showed that TPX2 showed very high expression in ccRCC, and patients with higher TPX2 expression had shorter relative survival. Low miRNA-27b-3p expression was found in ccRCC. Knockdown of TPX2 or forced expression of miRNA-27b-3p in ccRCC cells inhibited cell proliferation, migration, invasion, and arrested cell division in G₀/G₁ phase. Dual luciferase reporter presented that miRNA-27b-3p targeted TPX2 to inhibit its expression. Rescue experiments demonstrated that the miRNA-27b-3p/ TPX2 axis affected the biological functions of ccRCC cells. Concurrent overexpression of miRNA-27b-3p and TPX2 inhibited the facilitating effect of TPX2 on ccRCC cell growth. The results revealed novel regulatory mechanisms involved in ccRCC progression, hoping that it may spark an insight for later discovery about the new therapeutic targets for ccRCC.

中文翻译：

---

miRNA-27b-3p/TPX2 轴调节透明细胞肾细胞癌细胞增殖、侵袭和迁移

有多种癌细胞与 TPX2 的存在有关。然而，关于其在透明细胞肾细胞癌（ccRCC）的发展和维持中的作用的证据并不多。在我们的研究中，进行生物信息学分析以获得ccRCC中差异表达的mRNA和miR-NA。生存曲线预测 TPX2 表达与患者生存的相关性。通过数据库预测TPX2的上游调控miRNA为miRNA-27b-3p，双荧光素酶实验验证了靶向关系。采用qRT-PCR和Western blot检测ccRCC细胞中TPX2 mRNA和蛋白的表达。通过CCK-8、集落形成、伤口愈合、Transwell和流式细胞术检测增殖、侵袭、迁移和细胞周期。结果显示，TPX2在ccRCC中表现出非常高的表达，TPX2表达较高的患者相对生存期较短。在 ccRCC 中发现低 miRNA-27b-3p 表达。在ccRCC细胞中敲低TPX2或强制表达miRNA-27b-3p可抑制细胞增殖、迁移、侵袭，并将细胞分裂阻滞在G ₀ /G ₁期。双荧光素酶报告基因显示 miRNA-27b-3p 靶向 TPX2 抑制其表达。拯救实验表明，miRNA-27b-3p/TPX2轴影响ccRCC细胞的生物学功能。miRNA-27b-3p 和 TPX2 的同时过表达抑制了 TPX2 对 ccRCC 细胞生长的促进作用。结果揭示了参与 ccRCC 进展的新调控机制，希望它可以为后续发现 ccRCC 新治疗靶点激发见解。