Background

Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies and frequent cause of cancer-related death worldwide. Long non-coding RNAs (lncRNAs) play regulatory roles and serve as biomarkers of multiple cancers, including ESCC. Our previous studies have confirmed that lncRNA Kinectin 1 antisense RNA 1 (KTN1-AS1) is highly expressed in ESCC and exerts oncogene function through RBBP4/HDAC1 complex.

Objective

Our present study focused on exploring a novel molecular mechanism of KTN1-AS1 in ESCC.

Methods

In this study, qRT-PCR assay, Western blot assay, Luciferase reporter assay, and RNA immunoprecipitation assay were conducted.

Results

We found that KTN1-AS1 could bind to miR-885-5p in ESCC cells, and miR-885-5p was low expressed in ESCC. Overexpression of miR-885-5p inhibited esophageal cancer cells proliferation and invasion in vitro. Mechanistic analysis demonstrated that miR-885-5p specifically targeted striatin 3 (STRN3), and KTN1-AS1/miR-885-5p promoted the EMT process by Hippo pathway in STRN3/YAP1 dependent manner.

Conclusion

To sum up, KTN1-AS1 facilitates ESCC progression by acting as a ceRNA for miR-885-5p to regulate STRN3 expression and the Hippo pathway, and KTN1-AS1 maybe used as a promising therapeutic target for ESCC.

中文翻译：

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LncRNA KTN1-AS1通过miR-885-5p/STRN3轴促进食管鳞状细胞癌进展

背景

食管鳞状细胞癌（ESCC）是全世界最常见的恶性肿瘤之一，也是癌症相关死亡的常见原因。长非编码 RNA (lncRNA) 发挥调节作用，并可作为包括 ESCC 在内的多种癌症的生物标志物。我们前期的研究证实lncRNA Kinectin 1反义RNA 1（KTN1-AS1）在ESCC中高表达，并通过RBBP4/HDAC1复合物发挥癌基因功能。

客观的

我们目前的研究重点是探索 KTN1-AS1 在 ESCC 中的新分子机制。

方法

在本研究中，进行了 qRT-PCR 测定、Western blot 测定、荧光素酶报告基因测定和 RNA 免疫沉淀测定。

结果

我们发现KTN1-AS1在ESCC细胞中可以与miR-885-5p结合，并且miR-885-5p在ESCC中低表达。miR-885-5p 的过表达可抑制体外食管癌细胞的增殖和侵袭。机制分析表明，miR-885-5p特异性靶向striatin 3（STRN3），KTN1-AS1/miR-885-5p以STRN3/YAP1依赖性方式通过Hippo途径促进EMT过程。

结论

综上所述，KTN1-AS1通过作为miR-885-5p的ceRNA调节STRN3表达和Hippo通路来促进ESCC进展，并且KTN1-AS1可能作为ESCC的有前景的治疗靶点。