Combining protein skimming with ozone (O₃) is a common method for removing microparticles in recirculating aquaculture systems (RAS). Nevertheless, there is a limited number of studies that have validated protein skimming's performance at a commercial scale. Additionally, variations in protein skimmer designs and operational variables may yield different performance outcomes. In the present study, the performance of two types of full-scale protein skimmer (S1 and S2) were compared and evaluated under two levels of hydraulic retention time (HRT) (1.8 and 2.2 min) and three levels of O₃ doses (0, 7, and 14 g O₃/kg feed) in a commercial seawater RAS facility. Samples from the inlet and outlet of the protein skimmers were collected at each combination of operational variables. They were analysed for several relevant water quality parameters to quantify the treatment efficiency. O₃ dose significantly improved water quality and reduced the numbers of microparticles and bacterial activity in a single pass. Besides that, doses as high as 14 g O₃/kg feed significantly increased total residual oxidant (TRO) concentration. Additionally, an increase in HRT exerted a moderate effect on removing microparticles and a strong effect on redox potential (ORP) and TRO. Finally, the type of protein skimmer only affected the ORP, causing no significant changes to other water quality metrics. The correlations between the investigated water quality parameters defined a clear pattern of the ongoing processes and particle characteristics. Overall, the results demonstrated that protein skimming combined with carefully selected O₃ doses can improve general water quality and control critical factors such as bacterial activity and microparticles under commercial operations.

将蛋白质撇渣与臭氧 (O ₃ ) 相结合是去除循环水产养殖系统 (RAS) 中微粒的常用方法。然而，在商业规模上验证蛋白质脱脂性能的研究数量有限。此外，蛋白质分离器设计和操作变量的变化可能会产生不同的性能结果。在本研究中，对两种全尺寸蛋白质分离器（S1和S2）在两种水力停留时间（HRT）水平（1.8和2.2分钟）和三种O 3 剂量水平_（ 0 、7 和 14 g O ₃/kg 饲料）在商业海水 RAS 设施中。在每个操作变量组合下收集来自蛋白质分离器入口和出口的样品。对它们的几个相关水质参数进行了分析，以量化处理效率。O ₃剂量显着改善了水质，并减少了单次通过的微粒数量和细菌活性。除此之外，剂量高达 14 g O ₃/kg饲料显着增加总残留氧化剂（TRO）浓度。此外，HRT 的增加对去除微粒产生中等影响，对氧化还原电位 (ORP) 和 TRO 产生强烈影响。最后，蛋白质分离器的类型仅影响 ORP，不会对其他水质指标造成重大变化。所研究的水质参数之间的相关性定义了正在进行的过程和颗粒特征的清晰模式。总体而言，结果表明，蛋白质撇渣与精心选择的 O ₃剂量相结合可以改善总体水质并控制商业运营中的细菌活性和微粒等关键因素。