Population and genotype data are essential for genetic mapping. The multi-parent advanced generation intercross (MAGIC) population is a permanent mapping population used for precisely mapping quantitative trait loci. Moreover, genotyping-by-target sequencing (GBTS) is a robust high-throughput genotyping technology characterized by its low cost, flexibility, and limited requirements for information management and support. In this study, an 8-way MAGIC population was constructed using eight elite founder lines. In addition, GenoBaits Peanut 40K was developed and utilized for the constructed MAGIC population. A subset (297 lines) of the MAGIC population at the S2 stage was genotyped using GenoBaits Peanut 40K. Furthermore, these lines and the eight parents were analyzed in terms of pod length, width, area, and perimeter. A total of 27 single nucleotide polymorphisms (SNPs) were revealed to be significantly associated with peanut pod size-related traits according to a genome-wide association study. The GenoBaits Peanut 40K provided herein and the constructed MAGIC population will be applicable for future research to identify the key genes responsible for important peanut traits.

群体和基因型数据对于遗传作图至关重要。多亲先进代杂交（MAGIC）群体是一个永久作图群体，用于精确绘制数量性状基因座。此外，靶标基因分型测序（GBTS）是一种强大的高通量基因分型技术，其特点是成本低、灵活性强、对信息管理和支持的要求有限。在这项研究中，使用八个精英创始人系构建了 8 路 MAGIC 群体。此外，GenoBaits Peanut 40K 是为构建的 MAGIC 种群而开发和使用的。使用 GenoBaits Peanut 40K 对 S2 阶段 MAGIC 群体的子集（297 个系）进行基因分型。此外，还对这些品系和八个亲本的荚果长度、宽度、面积和周长进行了分析。根据全基因组关联研究，共有 27 个单核苷酸多态性 (SNP) 被发现与花生荚大小相关性状显着相关。本文提供的 GenoBaits Peanut 40K 和构建的 MAGIC 群体将适用于未来的研究，以确定负责重要花生性状的关键基因。