Plasmalemma vesicle associated protein (PLVAP) is commonly considered to be specifically expressed in endothelial cells in which it localized to diaphragms of caveolae, fenestrae, and transendothelial channels. PLVAP is reported to be an important regulator of heart development and a novel target to promote cardiac repair in the ischemic heart. However, the dynamics of plvap expression in heart development, homeostasis and pathology have not been comprehensively described. In this study, we analyzed the temporal and spatial expression of plvap in mouse heart under different conditions. We found that, during embryonic and neonatal stages, PLVAP was detected in endocardial endothelial cells, epicardial mesothelial cells, and a small amount of coronary vascular endothelial cells. In adult heart, PLVAP was also identified in endocardial cells and a few coronary vascular endothelial cells. However, epicardial expression of PLVAP was lost during postnatal heart development and cannot be detected in mouse heart by immunostaining since 3-week-old. We also analyzed the expression of plvap in a model of cardiac hypertrophy and failure induced by transverse aortic constriction surgery, and identified expression of PLVAP in endocardial cells and coronary vascular endothelial cells in the injured heart. This study provides new evidence to better understand the role of plvap in mouse heart development and injury.

质膜囊泡相关蛋白（PLVAP）通常被认为在内皮细胞中特异性表达，其定位于小窝隔膜、窗孔和跨内皮通道。据报道，PLVAP 是心脏发育的重要调节因子，也是促进缺血性心脏修复的新靶点。然而， plvap表达在心脏发育、稳态和病理学中的动态尚未得到全面描述。在本研究中，我们分析了不同条件下小鼠心脏中plvap的时空表达。我们发现，在胚胎和新生儿阶段，心内膜内皮细胞、心外膜间皮细胞和少量冠状血管内皮细胞中检测到PLVAP。在成人心脏中，心内膜细胞和少数冠状血管内皮细胞中也发现了 PLVAP。然而，PLVAP 的心外膜表达在出生后心脏发育过程中丢失，并且从 3 周龄起就无法通过免疫染色在小鼠心脏中检测到。我们还分析了横主动脉缩窄手术引起的心脏肥大和衰竭模型中PLVAP的表达，并鉴定了受损心脏的心内膜细胞和冠状血管内皮细胞中PLVAP的表达。这项研究为更好地了解plvap在小鼠心脏发育和损伤中的作用提供了新的证据。