Single-chain multicolor-reporter imaging templates were developed for the subcellular localization of molecular events in mammalian cells. The templates were constructed by tandem linkage of fluorescent protein variants – fused with luciferases and the subcellular localization signal peptides. The templates simultaneously reported steroid hormonal activities at different optical spectra in the subcellular compartments. The templates contribute to the expansion of a toolbox of optical probes for subcellular localization of molecular events in intact cells.

中文翻译：

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用于哺乳动物细胞中分子事件亚细胞定位的单链多色报告模板

单链多色报告基因成像模板是为哺乳动物细胞中分子事件的亚细胞定位而开发的。模板是通过荧光蛋白变体的串联连接构建的——与荧光素酶和亚细胞定位信号肽融合。这些模板同时报告了亚细胞区室中不同光谱下的类固醇激素活性。这些模板有助于扩展光学探针工具箱，用于完整细胞中分子事件的亚细胞定位。