Ocellated icefish (Chionodraco rastrospinosus, C. rastrospinosu), plays an essential role in the Antarctic food web, and is mainly distributed at depths of 200-1,000 m in the Southern Ocean. The abundance of this species has been drastically reduced due to various impacts, including overfishing and climate change. Therefore, in order to develop, sustainably exploit and protect this species, it is urgent to understand the state of its population resources. However, there is little information available regarding its genetic structure and a lack of effective molecular markers of this species. In this study, 105 novel single nucleotide polymorphism (SNP) markers were developed using double digest restriction site-associated DNA sequencing (ddRAD-Seq). The observed heterozygosity (H_o) and expected heterozygosity (H_e) ranged from 0 to 1 and 0.2188 to 0.5, respectively. The polymorphism information content (PIC) value ranged from 0.194824 to 0.375. After Bonferroni correction, none of the loci deviated significantly from Hardy-Weinberg equilibrium (p > 0.05). The development of these SNP markers will be useful for the genetic analysis and scientific management of C. rastrospinosus.

有眼冰鱼（Chionodraco rastrospinosus，C. rastrospinosu）在南极食物网中发挥着重要作用，主要分布在南大洋200-1,000米深处。由于过度捕捞和气候变化等各种影响，该物种的丰度已大幅减少。因此，为了开发、可持续利用和保护该物种，迫切需要了解其种群资源状况。然而，有关其遗传结构的信息很少，并且缺乏该物种的有效分子标记。在这项研究中，使用双消化限制性位点相关 DNA 测序 (ddRAD-Seq) 开发了 105 个新型单核苷酸多态性 (SNP) 标记。观察到的杂合度（H _o）和预期杂合度（He _）分别为 0 至 1 和 0.2188 至 0.5。多态性信息内容(PIC)值范围为0.194824至0.375。经过 Bonferroni 校正后，没有一个位点显着偏离 Hardy-Weinberg 平衡 ( p  > 0.05)。这些SNP标记的开发将有助于粗棘毛蝽的遗传分析和科学管理。