Diabetic nephropathy (DN) is a metabolic disease wherein chronic hyperglycemia triggers various renal cell dysfunctions, eventually leading to progressive kidney failure. Rosa laevigata Michx. is a traditional Chinese herbal medicine. Many studies have confirmed its antioxidative, anti-inflammatory, and renoprotective effects. However, the effects and mechanisms of Rosa laevigata Michx. polysaccharide (RLP) in DN remain unclear. In this study, a DN mouse model was established to investigate the therapeutic effect of RLP on DN mice. Then, nontargeted metabolomics was used to analyze the potential mechanism of RLP in the treatment of DN. Finally, the effects of RLP on ferroptosis and the PI3K/AKT pathway were investigated. The results demonstrated that RLP effectively alleviated renal injury and reduced inflammation and oxidative stress in the kidney. In addition, nontargeted metabolomic analysis indicated that RLP could modulate riboflavin metabolism and tryptophan metabolism in DN mice. Notably, ferroptosis and PI3K/AKT pathway-mediated apoptosis in the kidney were also ameliorated following RLP treatment. In conclusion, this study confirmed that RLP had a significant therapeutic effect on DN mice. Furthermore, RLP treatment modulated tryptophan metabolism and inhibited ferroptosis and PI3K/AKT pathway-mediated apoptosis in the kidney.

中文翻译：

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金樱子Michx。多糖通过抑制铁死亡和 PI3K/AKT 通路介导的细胞凋亡和调节色氨酸代谢来改善小鼠糖尿病肾病

糖尿病肾病（DN）是一种代谢性疾病，其中慢性高血糖引发各种肾细胞功能障碍，最终导致进行性肾衰竭。金樱子Michx。是一种传统的中草药。许多研究已证实其抗氧化、抗炎和肾脏保护作用。然而，金樱子的作用和机制。DN 中的多糖（RLP）尚不清楚。本研究建立DN小鼠模型，探讨RLP对DN小鼠的治疗作用。然后，利用非靶向代谢组学分析RLP治疗DN的潜在机制。最后，研究了 RLP 对铁死亡和 PI3K/AKT 通路的影响。结果表明，RLP有效减轻肾损伤，减少肾脏炎症和氧化应激。此外，非靶向代谢组学分析表明，RLP 可以调节 DN 小鼠的核黄素代谢和色氨酸代谢。值得注意的是，RLP 治疗后，肾脏中的铁死亡和 PI3K/AKT 通路介导的细胞凋亡也得到改善。总之，本研究证实RLP对DN小鼠具有显着的治疗作用。此外，RLP 治疗可调节色氨酸代谢并抑制肾脏中铁死亡和 PI3K/AKT 途径介导的细胞凋亡。